A0262
乙酰磷酸钾锂
≥85% (elemental analysis), powder
别名:
乙酰磷酸 锂钾盐
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关于此项目
线性分子式:
CH3COOP(O)(OK)(OLi)
化学文摘社编号:
分子量:
184.06
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
304-378-0
MDL number:
Beilstein/REAXYS Number:
5689718
产品名称
乙酰磷酸钾锂, high-energy phosphate donor
InChI
1S/C2H5O5P.K.Li/c1-2(3)7-8(4,5)6;;/h1H3,(H2,4,5,6);;/q;2*+1/p-2
InChI key
RLQMPLKXFIXRCV-UHFFFAOYSA-L
SMILES string
[Li+].[K+].CC(=O)OP([O-])([O-])=O
assay
≥85% (elemental analysis)
form
powder
mp
>300 °C (lit.)
solubility
water: 25 mg/mL, clear, colorless
storage temp.
−20°C
Quality Level
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Application
乙酰磷酸锂钾：
- 已在X射线晶体学中用作 CpxR（转录因子）底物，以浸泡CpxR受体结构域(CpxRRD)晶体实现磷酸化
- 可用作筛选研究中的磷酸盐(P)供体，结合多种酶从种羟基化合物中筛选各类天然和合成有机磷酸盐供体，改进磷酸单酯的酶促合成
- 已用于构建用于体外翻译反应的预混物A
Biochem/physiol Actions
乙酰磷酸钾锂通常用作乙酰磷酸的来源。因此，在磷酸酶催化的转磷酸化反应中，乙酰磷酸盐可作为无机低聚磷酸盐的适宜替代品，减少无机单磷酸盐副产物形成。
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Repr. 2 - Skin Corr. 1B
存储类别
8B - Non-combustible corrosive hazardous materials
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Ariel E Mechaly et al.
Structure (London, England : 1993), 25(6), 939-944 (2017-05-30)
Bacterial two-component systems consist of a sensor histidine kinase (HK) and a response regulator (RR). HKs are homodimers that catalyze the autophosphorylation of a histidine residue and the subsequent phosphoryl transfer to its RR partner, triggering an adaptive response. How
Soma Mukhopadhyay et al.
Bioorganic chemistry, 36(2), 65-69 (2008-02-26)
Acetate kinase, a member of the ASKHA (Acetate and Sugar Kinases, Hsp70, Actin) phosphotransferase superfamily is a central enzyme in prokaryotic carbon and energy metabolism. Recently extensive structural and biochemical studies of acetate kinase and related carboxylate kinases have been
Gábor Tasnádi et al.
Advanced synthesis & catalysis, 360(12), 2394-2401 (2018-10-20)
Undesired product hydrolysis along with large amounts of waste in form of inorganic monophosphate by-product are the main obstacles associated with the use of pyrophosphate in the phosphatase-catalyzed synthesis of phosphate monoesters on large scale. In order to overcome both
Sebastian R Schmidl et al.
Nature chemical biology, 15(7), 690-698 (2019-05-22)
Two-component systems (TCSs) are the largest family of multi-step signal transduction pathways and valuable sensors for synthetic biology. However, most TCSs remain uncharacterized or difficult to harness for applications. Major challenges are that many TCS output promoters are unknown, subject
Birgit Dreier et al.
Methods in molecular biology (Clifton, N.J.), 687, 283-306 (2010-10-23)
The selection and concomitant affinity maturation of proteins to bind to user-defined target molecules have become a key technology in biochemical research, diagnostics, and therapy. One of the most potent selection technologies for such applications is ribosome display. It works
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