产品名称
抗-小鼠IgG(Fc特异性)-过氧化物酶 山羊抗, affinity isolated antibody
biological source
goat
conjugate
peroxidase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
species reactivity
mouse
technique(s)
direct ELISA: 1:50,000
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
western blot: 1:80,000-1:160,000 using total cell extract of HeLa cells (5-10 μg per well
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
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Application
山羊抗小鼠IgG (Fc特定)-过氧化物酶抗体用于:
- 免迹印迹
- 酶联免疫吸附测定(ELISA)和
- 间接免疫过氧化物酶测定(IIPA)
通过使用生物合成PND和链霉亲和素涂层板进行ELISA试验,从而对免疫小鼠血液中一种新的PND抗体的特异性进行检测。HRP缀合的山羊抗小鼠IgG(Fc特异性)可用作二抗。
Disclaimer
除非我们的产品目录或产品随附的其他公司文档中另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人或动物。
General description
免疫球蛋白G(IgG)是一种糖蛋白抗体,其可调节免疫反应,如吞噬作用,也可参与自身免疫疾病的发展。小鼠IgG具有四种不同的同种型,即IgG1、IgG2a、IgG2b和IgG3。IgG1可调节小鼠的补体结合。
通过免疫特异性纯化从山羊抗小鼠IgG抗血清中分离抗体,该过程可基本上去除所有山羊血清蛋白,包括免疫球蛋白(其不可与小鼠IgG的Fc片段特异性结合)。以0.2%戊二醛为交联剂,将抗鼠IgG与过氧化物酶偶联。该抗体制剂采用人IgG吸附固相制备,以确保组织或细胞制剂中的交叉反应性最低。
通过免疫特异性纯化从山羊抗小鼠IgG抗血清中分离抗体,该过程可基本上去除所有山羊血清蛋白,包括免疫球蛋白(其不可与小鼠IgG的Fc片段特异性结合)。以0.2%戊二醛为交联剂,将抗鼠IgG与过氧化物酶偶联。该抗体制剂采用人IgG吸附固相制备,以确保组织或细胞制剂中的交叉反应性最低。
Immunogen
纯化的小鼠IgG Fc片段
Physical form
0.01M磷酸缓冲盐缓冲液(pH7.4, 含有0.05% MIT)溶液。
Preparation Note
通过Avrameas, S., et al., Scand.J. Immunol., 8, Suppl. 7, 7 (1978)等描述的两步戊二醛方法进行制备。
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signalword
Warning
hcodes
Hazard Classifications
Skin Sens. 1
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
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