产品名称
SYBR® Green JumpStart™ Taq ReadyMix™ 用于定量PCR，毛细管配制, SYBR® Green qPCR reagent for Roche LightCycler® capillary systems
form
liquid
usage
sufficient for 100 reactions
sufficient for 400 reactions
feature
dNTPs included
hotstart
concentration
1 units/reaction (20 μL reaction volume)
technique(s)
qPCR: suitable
color
colorless
input
purified DNA
compatibility
for use with Roche LightCycler 480
detection method
SYBR® Green
shipped in
wet ice
storage temp.
−20°C
Application
Biochem/physiol Actions
JumpStart Taq抗体在室温下能够使DNA聚合酶失活。当温度在第一步循环变性中升高到70℃以上，复合体解离，聚合酶完全被激活。JumpStart Taq DNA聚合酶防止非特异性扩增，能够获得更为准确的CT值。
为制备反应，将10 μL ReadyMix加入引物、模板和水中，使最终反应体积为20 μL。
Features and Benefits
- 方便的2×浓缩预混液，专门设计用于毛细管仪器，例如Roche LightCycler®，是高通量应用的理想选择
- 提高特异性&目标产量-JumpStart™ Taq聚合酶可防止非特异性产物，从而获得更准确的CT值并改善定量
- 该预混液可确保反应间一致性
- 缩短了制备时间，并降低了多个移液步骤造成的污染风险
- SYBR® Green I荧光染料特异性结合双链DNA，低价、易用、灵敏，是任意DNA序列定量分析的理想选择
General description
SYBR Green Taq ReadyMix可用于单个产品实时扩增实验，也可用于评价产生荧光标记探针之前的引物序列。 不建议将荧光标记探针与SYBR Green I染料一起使用。
Legal Information
Other Notes
Packaging
100RXN包装为1 X 1 mL
400RXN包装为1 X 4 mL
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
商品
The polymerase chain reaction is one of the most widely used techniques in molecular biology. The PCR process consists of three main steps, Denaturation, Annealing & Extension
Watch these videos to learn how real time or quantitative PCR (qPCR) works and the benefits of both the SYBR Green-based and probe-based methods of qPCR assay.
qPCR investigates gene expression, amplification, and alterations, crucial for tumor biology and understanding cancer genetics.
聚合酶链反应是分子生物学应用最普遍的技术之一。PCR过程主要由三步组成：变性、退火和延伸
实验方案
A protocol that can be used as a basic template for qPCR incorporating SYBR Green I DNA binding dye that is amenable to modification and applicable for use as validation for a set of primers.
Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions
我们的 SYBR Green qPCR 协议是专为检测基因表达和 RT-PCR 反应的精确定量而设计的方法。
相关内容
RT-qPCR detects specific targets with applications in gene expression and pathogen detection.
SYBR® Green I, a commonly used fluorescent DNA binding dye, binds all double-stranded DNA and detection is monitored by measuring the increase in fluorescence throughout the cycle. Explore our LuminoCt® and KiCqStart® products for Fast qPCR or JumpStart™ reagents for conventional qPCR
Instructions
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