usage
kit sufficient for 50 reactions
Quality Level
analyte chemical class(es)
proteins (FLAG® Affinity Gels)
technique(s)
immunoprecipitation (IP): suitable
shipped in
wet ice
storage temp.
−20°C
General description
Sigma的FLAG免疫沉淀试剂盒可快速、高效地免疫沉淀和洗脱FLAG标记蛋白。免疫沉淀过程使用ANTI-FLAG M2亲和凝胶进行,它是一种与琼脂糖树脂共价连接的高特异性单克隆抗体。亲和树脂可使FLAG标记蛋白的结合高效进行,而无需预备步骤或校准。免疫沉淀后的FLAG标记蛋白可以在酸性条件下或使用竞争性FLAG肽,从树脂上高效地洗脱下来。免疫沉淀后的蛋白可用于电泳或其它活性检测方法,以检测它们的大小、翻译后修饰及相互作用。
Application
更多产品信息,请访问我们的FLAG®应用页面。
Features and Benefits
- 利用高特异性的抗-FLAG M2亲和凝胶
- 无需预备步骤和校准
- 3xFLAG™肽通过直接竞争法实现高效温和的洗脱
Other Notes
所有步骤在2-8°C进行。
Legal Information
3xFLAG is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
10 - Combustible liquids
wgk
WGK 3
signalword
Danger
hcodes
Hazard Classifications
Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2
法规信息
低风险生物材料
常规特殊物品
此项目有
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Human respiratory syncytial virus (RSV), a major cause of severe respiratory diseases, efficiently suppresses cellular innate immunity, represented by type I interferon (IFN), using its two unique nonstructural proteins, NS1 and NS2. In a search for their mechanism, NS1 was
Mateusz Wydro et al.
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Mammalian mitochondria contain their own genome that is almost fully transcribed from both strands, generating polycistronic RNA units that are processed and matured. The mitochondrial mRNA is modified by oligo- or polyadenylation at the 3' termini, but the exact function
Andrew S Terker et al.
American journal of physiology. Renal physiology, 315(4), F781-F790 (2018-02-08)
With no lysine kinase 4 (WNK4) is essential to activate the thiazide-sensitive NaCl cotransporter (NCC) along the distal convoluted tubule, an effect central to the phenotype of familial hyperkalemic hypertension. Although effects on potassium and sodium channels along the connecting
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| FLAGIPT1-1KT | 04061833622902 |
