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Merck
CN

96013

Sigma-Aldrich

Abberior® STAR 635, maleimide

for STED application

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UNSPSC代码:
12352200
NACRES:
NA.32

检测方案

≥80.0%

形式

solid

溶解性

DMF: 0.25 mg/mL, clear

荧光

λex 640 nm; λem 655 nm±10 nm in PBS, pH 7.4

储存温度

−20°C

一般描述

Absorption Maximum, λmax: 635 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 110,000 M-1cm-1 (PBS, pH 7.4)
Correction Factor, CF260 = ε260/εmax: 0.26 (PBS, pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0.38 (PBS, pH 7.4)
Fluorescence Maximum, λfl: 656 nm (aq. acetonitrile; MeOH)
655 nm (PBS, pH 7.4)
Recommended STED Wavelength, λSTED: 750 −780 nm
Fluorescence Quantum Yield, η: 0.88 (PBS, pH 7.4)
Fluorescence Lifetime, τ: 2.8 (PBS, pH 7.4)

应用

  • Abberior Star 635 maleimide has been used for single-molecule FRET (Förster resonance energy transfer) spectroscopy for protein dynamics in live cells.
  • Abberior STAR 635 goat anti-rabbit antibody has been used for STED (stimulated emission depletion) imaging of primary cultured striatal neurons obtained from rats.
  • Abberior STAR 635 anti-rabbit antibody has been used for STED imaging of non-sensory supporting cells obtained from cochlea of rats and mice.
  • Abberior STAR 635 conjugated with secondary antibody has been used for STED imaging of microtubules in cells.

适用性

Designed and tested for fluorescent super-resolution microscopy

法律信息

6538 is a trademark of American Type Culture Collection
abberior is a registered trademark of Abberior GmbH

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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PloS one, 8(9), e75155-e75155 (2013-09-24)
The phosphoprotein DARPP-32 (dopamine and cyclic adenosine 3´, 5´-monophosphate-regulated phosphoprotein, 32 kDa) is an important component in the molecular regulation of postsynaptic signaling in neostriatum. Despite the importance of this phosphoprotein, there is as yet little known about the nanoscale
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Journal of biomedical optics, 19(10), 105003-105003 (2014-10-08)
Atomic force microscopes (AFM) provide topographical and mechanical information of the sample with very good axial resolution, but are limited in terms of chemical specificity and operation time-scale. An optical microscope coupled to an AFM can recognize and target an
Arnaud P Giese et al.
Development (Cambridge, England), 139(20), 3775-3785 (2012-09-20)
Vangl2 is one of the central proteins controlling the establishment of planar cell polarity in multiple tissues of different species. Previous studies suggest that the localization of the Vangl2 protein to specific intracellular microdomains is crucial for its function. However
T A Klar et al.
Optics letters, 24(14), 954-956 (2007-12-13)
We overcame the resolution limit of scanning far-field fluorescence microscopy by disabling the fluorescence from the outer part of the focal spot. Whereas a near-UV pulse generates a diffraction-limited distribution of excited molecules, a spatially offset pulse quenches the excited
Tim Grotjohann et al.
Nature, 478(7368), 204-208 (2011-09-13)
Lens-based optical microscopy failed to discern fluorescent features closer than 200 nm for decades, but the recent breaking of the diffraction resolution barrier by sequentially switching the fluorescence capability of adjacent features on and off is making nanoscale imaging routine. Reported

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