等级
for molecular biology
质量水平
产品线
BioReagent
形式
powder
适用性
in accordance for ribonuclease inhibition test
运输
wet ice
储存温度
−20°C
一般描述
钒基核糖核苷复合物是核糖核酸酶的低分子量抑制剂。
应用
钒基核糖核苷复合物已被用于杂交混合物用于RNA原位杂交。
钒基核糖核苷复合物（VDR）是一类过渡态类似物，可与多种RNase的活性位点结合而抑制其活性。由于RNase不会被复合物进行共价修饰，因此VDR必须被用于RNA提取和纯化的每个阶段。然而，VDR还可抑制RNA聚合酶和体外翻译，因此必须将其从最终RNA制备产物中去除。
生化/生理作用
钒基核糖核苷复合物在金黄色葡萄球菌中具有阻断核糖体亚基形成的能力。该复合物具有抗微生物活性 这些复合物也能作为多种不带有核糖核酸酶活性的酶抑制剂。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
Isolation of cytoplasmic RNA: ribonucleoside-vanadyl complexes.
S L Berger
Methods in enzymology, 152, 227-234 (1987-01-01)
Analysis of TMEM174 gene expression in various renal cancer types by RNA in situ hybridization
Zhang X, et al.
Oncology Letters, 8(4), 1693-1696 (2014)
G Rotondo et al.
Nucleic acids research, 24(12), 2377-2386 (1996-06-15)
The pac1+ gene of the fission yeast Schizosaccharomyces pombe is essential for viability and its overexpression induces sterility and suppresses mutations in the pat1+ and snm1+ genes. The pac1+ gene encodes a protein that is structurally similar to RNase III
R Boom et al.
Journal of clinical microbiology, 28(3), 495-503 (1990-03-01)
We have developed a simple, rapid, and reliable protocol for the small-scale purification of DNA and RNA from, e.g., human serum and urine. The method is based on the lysing and nuclease-inactivating properties of the chaotropic agent guanidinium thiocyanate together
Effect of ribonucleoside-vanadyl complexes on enzyme-catalyzed reactions central to recombinant DNA technology
Puskas R S, et al.
Biochemistry, 21(19), 4602-4608 (1982)
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