88417
MTT 甲臜
MTT reduction end product, powder
别名:
1-（4，5-二甲基吡啶-2-基）-3，5-二苯基甲酰胺, 噻唑基蓝甲臜
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关于此项目
经验公式（希尔记法）:
C18H17N5S
化学文摘社编号:
分子量:
335.43
MDL编号:
UNSPSC代码:
12352204
PubChem化学物质编号:
NACRES:
NA.83
产品名称
MTT 甲臜, MTT reduction end product
质量水平
表单
powder
溶解性
chloroform: 10 mg/mL, clear, dark violet
SMILES字符串
Cc1nc(\N=N\C(=N\Nc2ccccc2)c3ccccc3)sc1C
InChI
1S/C18H17N5S/c1-13-14(2)24-18(19-13)23-22-17(15-9-5-3-6-10-15)21-20-16-11-7-4-8-12-16/h3-12,20H,1-2H3/b21-17+,23-22+
InChI key
GIFKVQOPAJDJLO-SZVLODPRSA-N
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应用
MTT甲臜用于细胞活率检测。
生化/生理作用
MTT法是一种比色反应分析方法，常用于检测细胞活率。该方法要求将溶于水的黄色3-[4,5-二甲基噻唑-2-yl]-2,5-基四氮唑嗅盐(MTT)酶促还原为不溶于水的紫色MTT-甲臜。该反应由线粒体脱氢酶催化，仅在活细胞中发生。因此，蓝色甲臜量与活细胞数量成正比。通过分光光度法分析570 nm波长处的甲臜溶液吸光度。
其他说明
评审：四唑盐和甲瓒
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
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