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表单
powder
质量水平
质量
4×cryst.
比活
~70 U/mg (acc. to Kunitz)
分子量
~13,700
Mr ~13700
杂质
proteases, none detected
运输
wet ice
储存温度
−20°C
SMILES字符串
[nH]1cnc(c1)CC(NC(=O)CCN)C(=O)O
InChI
1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
InChI key
CQOVPNPJLQNMDC-UHFFFAOYSA-N
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一般描述
RNase A（核糖核酸酶A）是一种内切核糖核酸酶，在嘧啶核苷酸后裂解单链RNA的磷酸二酯键。它可切割3′磷酸基末端（例如，pG-pG-pC-pA-pG将切割为pG-pG-pCp 和A-pG）。对单链RNA表现出最高活性。RNase A是含有四个二硫键的单链多肽。它与RNase B不同，并非糖蛋白。核糖核酸酶不会水解DNA，因为DNA缺乏形成环状中间体所必需的2′-OH基团。RNase A还可以水解蛋白质样品中的RNA。RNase A可被His12和His119的烷基化抑制并被钾盐和钠盐活化。RNAse在重金属离子存在时受到抑制。此外，RNase也被DNA竞争性抑制。
应用
- RNase A用于去除DNA质粒和基因组DNA制品和蛋白质样品中的RNA。
- RNase A还用于RNA序列分析和保护测定。
- RNase A已用作计算辅助药物设计的工具。
- RNase A为RNA序列分析提供支持。
- RNase A水解蛋白质样品中的RNA。
- RNase A为DNA纯化提供支持。
特点和优势
我们高度稳定的核糖核酸酶A——RNase A，适合于RNA去除、RNA测序和DNA纯化。
单位定义
1 U corresponds to the amount of enzyme which hydrolyzes the RNA at a rate constant k = 1 at 25°C and pH 5.0 (Kunitz-units); M. Kunitz, J. Biol. Chem. 164, 563 (1946)
分析说明
蛋白测定方法：E.
其他说明
Sales restrictions may apply
The enzyme only hydrolyzes phosphodiester linkages which originate from pyrimidine-3′-phosphates, as well as a large number of 2′,3′-cyclo compounds of cytidine and uridine derivatives; The return of pancreatic ribonucleases
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
[On the mechanism of the ribonuclease reaction. 1. The function of the pyrimidine base in the reaction].
H G Gassen et al.
European journal of biochemistry, 1(1), 36-45 (1967-03-01)
S A Benner et al.
Trends in biochemical sciences, 14(10), 396-397 (1989-10-01)
A decade after losing favor as an 'uninteresting' digestive enzyme, pancreatic ribonuclease has been found to be homologous to a series of extracellular proteins that may influence tumor cell growth, neurological development and biological differentiation. One surprising outcome of these
Caterina Brandmayr et al.
Angewandte Chemie (International ed. in English), 51(44), 11162-11165 (2012-10-06)
Useful diversity: Quantification of modified tRNA nucleobases in different murine and porcine tissues reveals a tissue-specific overall modification content. The modification content correlates with rates of protein synthesis in vitro, suggesting a direct link between tRNA modification levels and tissue-specific translational
Pinaki P Misra et al.
Biopolymers, 97(12), 933-949 (2012-09-19)
In this study, we extensively report the effect of glycine betaine during the refolding of partially folded bovine α-lactalbumin (α-LA) in presence of hexadecyl trimethyl ammonium bromide (HTAB), and Ribonuclease A (RNAse A) in presence of sodium dodecyl sulfate (SDS)
Shuangsheng Huang et al.
International journal of molecular medicine, 30(6), 1410-1416 (2012-10-03)
Tumor cells trigger angiogenesis through overexpression of various angiogenic factors including vascular endothelial growth factor (VEGF) and angiopoietin 1 (Ang1). Therefore, inhibition of the expression of both VEGF and Ang1, the initial step of tumor angiogenesis, is a promising strategy
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