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Merck
CN

83702

Sigma-Aldrich

罗丹明123

BioReagent, for fluorescence, ≥85% (HPLC)

别名:

2-(6-氨基-3-亚氨基-3H-呫吨-9-基)苯甲酸甲酯

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About This Item

线性分子式:
C21H17ClN2O3
CAS号:
分子量:
380.82
Beilstein:
6030951
EC 号:
MDL编号:
UNSPSC代码:
12352116
PubChem化学物质编号:
NACRES:
NA.32

等级

for fluorescence

质量水平

产品线

BioReagent

检测方案

≥85% (HPLC)

形式

powder

杂质

≤10% water

溶解性

ethanol: 20 mg/mL, clear, red

适用性

in accordance for fluorescence

SMILES字符串

Cl[H].COC(=O)c1ccccc1C2=C3C=CC(=N)C=C3Oc4cc(N)ccc24

InChI

1S/C21H16N2O3.ClH/c1-25-21(24)15-5-3-2-4-14(15)20-16-8-6-12(22)10-18(16)26-19-11-13(23)7-9-17(19)20;/h2-11,22H,23H2,1H3;1H

InChI key

MYFATKRONKHHQL-UHFFFAOYSA-N

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一般描述

罗丹明123是荧光染料。

应用

罗丹明123用于:
  • 作为P-糖蛋白(P-gp)的底物,可在荧光积累测定中读出功能性转运体活性
  • 标记细胞,通过流式细胞术估计P-gp的表达情况
  • 在转运研究中,添加至顶端到基底外侧(AB-转运)或基底外侧到顶端(BA-转运)
  • 检测线粒体膜电位(ΔΨm)

生化/生理作用

罗丹明123通常作为官能报告物,报告流式细胞术中的P-糖蛋白(P-gp)。
最常用于流式细胞术的荧光染料,可用作Pgp的功能性报告分子。在癌症治疗中,MDR蛋白的功能测定是比MDR蛋白表达水平更好的预后指标。Rh 123可用于多参数分析而无荧光干扰,可与常用蛋白质标记染料组合使用,如PE-Cy5和AMCA(7-氨基-4-甲基香豆素-3-乙酸)。近期报道表明,Rh123也可能是MRP1的底物。

其他说明

上活体线粒体特异性染料

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What are the excitation and emission wavelengths for Rhodamine 123 (Product Nos. 83702 and R8004)?

    Using ethanol as the solvent, the excitation maximum is  511 nm and the emission maximum is 534 nm.  The recommended excitation and emission wavelengths in PBS are 488 and 515-575 nm, respectively.

  6. Why would one select Rhodamine 123 (Product Nos. 83702 and R8004) instead of other rhodamine compounds?

    Due to is comparatively low toxicity, Rhodamine 123 is the dye of choice for visualizing mitochondria or for cell viability measurements.

  7. What is the solubility of Rhodamine 123, (Product Nos. 83702 and R8004)?

    Rhodamine 123 is soluble in ethanol at 20 mg/mL, and has been reported soluble in ether, various fats and lipids, and has limited solubility in water (no concentration given).

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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