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安全信息

79454

Sigma-Aldrich

Ampliflu Red Westernblot Kit

for fluorescent detection

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About This Item

UNSPSC代码:
12352200

用途

sufficient for 50 applications

荧光

λex 571 nm; λem 585 nm

储存温度

2-8°C

一般描述

<p>find more information here</p>

应用

The Ampliflu Red Western Blot Kit provides an alternative to the classical chemiluminescence detection. When working with Ampliflu Red, standard protocols for the immunoblotting-procedure can be used until the detection step. No extra time for signal accumulation is necessary as it is for chemiluminescent signals. The use of Ampliflu Red is suitable for protein quantities between 1 ng and 1 μg per band of protein.

原理

The kit contains the peroxidase substrate Ampliflu Red that is enzymatically converted to the highly fluorescent compound, resorufin. Fluorescent imaging is then performed on the immunoblot.

分析说明

Sensitivity: Limit of detection is 1 ng/band, but may vary depending on the antibodies used.

法律信息

Ampliflu is a trademark of Sigma-Aldrich Co. LLC

仅试剂盒组分

产品编号
说明
  • Dimethyl sulfoxide, puriss. p.a., ACS reagent, ≥99.9% (GC) 3 mL

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • 90101Ampliflu™ Red, for fluorescence, ≥98.0% (HPLC) 5 vial(s)化学品安全说明书
  • 79382Phosphate buffered saline, tablet 5 tablets化学品安全说明书

象形图

Exclamation mark
GHS07

警示用语：

Warning

危险声明

H302

危险分类

Acute Tox. 4 Oral

储存分类代码

10 - Combustible liquids

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

新产品

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分析证书(COA)

Lot/Batch Number
BCBJ0714V

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. How should Product 79454, Ampliflu™ Red Western Blot Kit, be used?

    See the product information sheet for this product (under Documents, above).

  6. What is the stability of Product 79454, Ampliflu™ Red Western Blot Kit, after reconstitution?

    Upon dissolution in DMSO, the Ampliflu red should be stored at 4 deg C. in the dark. It is stable for 1 month. Re-use of the staining solution is not recommended.

  7.  How can background staining be reduced when using Product 79454, Ampliflu™ Red Western Blot Kit?

    An optimal signal to background ratio may be obtained by using a low fluorescence PVDF membrane and a 5% (w/v) BSA blocking solution.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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