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Merck
CN

73737

Sigma-Aldrich

4-硝基苯磷酸盐 双(三羟甲基氨基甲烷) 盐

phosphatase substrate, ≥97.0% (enzymatic), powder or crystals

别名:

p-Nitrophenylphosphate bis(tris) salt, p-nitrophenyl phosphate bis(tris) salt, PNPP, PNPP bis(tris) salt

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About This Item

线性分子式:
(C6 H4 N O6 P)( C4 H12 N O3)2
CAS号:
分子量:
461.36
Beilstein:
1578142
EC 号:
MDL编号:
UNSPSC代码:
12352204
PubChem化学物质编号:
NACRES:
NA.32

产品名称

4-硝基苯磷酸盐 双(三羟甲基氨基甲烷) 盐, for the determination of phosphatase, ≥97.0% (enzymatic)

质量水平

方案

≥97.0% (enzymatic)

表单

powder or crystals

质量

for the determination of phosphatase

杂质

≤0.1% free nitrophenol
≤6% water

溶解性

H2O: 0.1 g/mL, clear

储存温度

2-8°C

SMILES字符串

NC(CO)(CO)CO.NC(CO)(CO)CO.OP(O)(=O)Oc1ccc(cc1)[N+]([O-])=O

InChI

1S/C6H6NO6P.2C4H11NO3/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;2*5-4(1-6,2-7)3-8/h1-4H,(H2,10,11,12);2*6-8H,1-3,5H2

InChI key

XXAXKCWOTRABOW-UHFFFAOYSA-N

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相关类别

应用

4-硝基苯磷酸盐 双(三羟甲基氨基甲烷)盐被用于测定细胞裂解后酸性和碱性磷酸酶的酶活性。

生化/生理作用

4-硝基苯磷酸盐是一种非蛋白质和非特异性的显色底物, 是酶联免疫吸附试验(ELISA)中碱性磷酸酶(ALP)的首选。它广泛应用于测定蛋白酪氨酸磷酸酶(PTPs)。酶切后,底物产生黄色产物,可以采用分光光度计测定。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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访问文档库

Elif Özlem Arslan-Aydoğdu et al.
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 49(1), 189-199 (2017-10-19)
Nine Legionella pneumophila strains isolated from cooling towers and a standard strain (L. pneumophila serogroup 1, ATCC 33152, Philadelphia 1) were analyzed and compared in terms of motility, flagella structure, ability to form biofilms, enzymatic activities (hemolysin, nucleases, protease, phospholipase
Ulrike Lorenz
Current protocols in immunology, Chapter 11, Unit 11-Unit 11 (2011-04-05)
Tyrosine phosphorylation and dephosphorylation of proteins play a critical role in many processes of the immune system, from early development to fully differentiated effector function. Since the opposing actions of protein tyrosine kinases (PTKs) and protein tyrosine phosphatases (PTPs) determine
Enzyme-linked Immunosorbent Assay (ELISA): From A to Z null
D.W. Moss
Methods of Enzymatic Analysis, 92-92 null
O.H. Lowry
Methods in Enzymology, 4, 366-366 (1957)

商品

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

硝基蓝四唑(NBT)可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐(BCIP)一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物,其颜色为蓝至紫色,可以通过视觉观察到。

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