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形式
solid
浓度
≥50.0% (degree of coupling)
溶解性
DMF: 1 mg/mL, clear
荧光
λex 510 nm; λem 514-534 nm in PBS, pH 7.4
储存温度
−20°C
一般描述
Abberior STAR 488是STED显微镜的最新发展。它是一种非常明亮的绿色荧光染料，可以在主要的488 nm激光波长上非常有效地被激发。Abberior STAR 488可以替代Oregon绿488、Atto™ 488或Alexa Fluor 488等染料。
STED：在STED显微镜中可在585-605 nm处最有效地耗尽染料。
Abberior STAR 488是可在488 nm处激发的染料。并且，该染料专门设计和测试与我们的STAR 440Sx（在590 nm处激发）进行联合使用，用于双色STED显微镜中。该染料已在Leica 双色TCS STED CW显微镜中成功测试。
关键特性
最大吸收波长，λmax: 503 nm (PBS, pH 7.4)
消光系数，ε(λmax): 65′000 M-1cm-1 (PBS, pH 7.4)
校正系数，CF260 = ε260/εmax: 0.28 (PBS, pH 7.4)
校正系数 CF280 = ε280/εmax: 0.14 (PBS, pH 7.4)
荧光最大值，λfl: 524nm (PBS, pH 7.4)
推荐的STED波长，λSTED: 585 - 605 nm
荧光量子产率，η: 0.89 (PBS, pH 7.4)
荧光寿命，τ: 3.9 ns (MeOH)
STED：在STED显微镜中可在585-605 nm处最有效地耗尽染料。
Abberior STAR 488是可在488 nm处激发的染料。并且，该染料专门设计和测试与我们的STAR 440Sx（在590 nm处激发）进行联合使用，用于双色STED显微镜中。该染料已在Leica 双色TCS STED CW显微镜中成功测试。
关键特性
- 可在488 nm激发的STED染料
- 在STED显微镜中的理想耗尽行为（〜590 nm）
- STAR 440SX的双色标记组合，可用于双色STED显微镜
最大吸收波长，λmax: 503 nm (PBS, pH 7.4)
消光系数，ε(λmax): 65′000 M-1cm-1 (PBS, pH 7.4)
校正系数，CF260 = ε260/εmax: 0.28 (PBS, pH 7.4)
校正系数 CF280 = ε280/εmax: 0.14 (PBS, pH 7.4)
荧光最大值，λfl: 524nm (PBS, pH 7.4)
推荐的STED波长，λSTED: 585 - 605 nm
荧光量子产率，η: 0.89 (PBS, pH 7.4)
荧光寿命，τ: 3.9 ns (MeOH)
应用
抗兔IgG抗体® STAR 488抗体已作为二抗用于：
- 在前列腺癌细胞系PPC-1和TSU-Pr1中进行荧光免疫组化分析
- 用于HeLa和猴肾COS-7细胞的免疫荧光中
- OLN-t40-α-syn细胞（少突胶质细胞）中的STED（刺激发射损耗）显微镜分析
适用性
设计和测试用于荧光超分辨率显微镜
其他说明
法律信息
Atto is a trademark of Atto-Tec GmbH
abberior is a registered trademark of Abberior GmbH
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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