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Merck
CN
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文件

47989

Sigma-Aldrich

Fura 2-AM

suitable for fluorescence, BioReagent, ≥95.0% (HPLC)

别名:

Fura 2 五（乙酰氧基甲基）酯

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About This Item

经验公式（希尔记法）:
C44H47N3O24
CAS号:
108964-32-5
分子量:
1001.85
Beilstein:
8183748
MDL编号:
MFCD00036976
UNSPSC代码:
12171500
PubChem化学物质编号:
57651141
NACRES:
NA.32

产品线

BioReagent

质量水平

100

检测方案

≥95.0% (HPLC)

形式

solid

溶解性

DMSO: soluble

荧光

λex 355 nm; λem 495 nm in 0.1 M Tris pH 8.0, esterase; 10 mM Ca2+

适用性

suitable for fluorescence

储存温度

−20°C

SMILES字符串

CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)c1ccc(C)cc1OCCOc2cc3cc(oc3cc2N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)-c4ncc(o4)C(=O)OCOC(C)=O

InChI

1S/C44H47N3O24/c1-25-7-8-32(46(16-39(53)65-20-60-26(2)48)17-40(54)66-21-61-27(3)49)35(11-25)58-9-10-59-36-12-31-13-37(43-45-15-38(71-43)44(57)69-24-64-30(6)52)70-34(31)14-33(36)47(18-41(55)67-22-62-28(4)50)19-42(56)68-23-63-29(5)51/h7-8,11-15H,9-10,16-24H2,1-6H3

InChI key

VPSRLGDRGCKUTK-UHFFFAOYSA-N

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相关类别

一般描述

Fura 2- am（Fura 2），又称乙酰氧基甲酯，是一种钙特定比率荧光染料。为了响应钙离子，像Fura 2这样的比例度量染料改变了各自的激发或发射光谱。细胞内钙浓度由不同波长的荧光发射比或激发决定。Fura 2的发射峰在505nm，激发峰在340-380nm之间，是首选的钙指示剂。

应用

Fura 2-AM是一种常用的探针，用于测定完整活细胞中细胞质游离钙的动态变化。

特点和优势

使用Fura 2的酯形式作为Fura 2-AM的主要好处有：

  • 可渗透细胞。
  • 容易载入细胞中。
与单波长染料相比，使用 Fura 2-AM 的另一个优点是，比率信号与以下因素无关：
  • 染料浓度。
  • 照明强度、
  • 光程长度。

其他说明

Fura-2的膜渗透性衍生物。用于将螯合剂渗透到细胞中。在细胞中发生酶促水解，产生Fura-2并保留在细胞内

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

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The use of microinjection and video microscopy for the study of calmodulin and calcium in living cells.
M Shelanski et al.
Methods in enzymology, 139, 824-834 (1987-01-01)
W Almers et al.
FEBS letters, 192(1), 13-18 (1985-11-11)
The Ca concentration ([Ca2+]i) in single rat peritoneal mast cells was measured by means of the new fluorescent Ca-indicator dye fura-2. Dye-loaded cells were made to degranulate with either antigen or compound 48/80. In cells loaded with extracellularly applied, membrane-permeant
Robin R Hodges et al.
Experimental eye research, 103, 99-113 (2012-09-15)
The purpose of this study was to identify the signaling pathways that epidermal growth factor (EGF) uses to stimulate mucin secretion from cultured rat conjunctival goblet cells and to compare the pathways used by EGF with those used by the
Rubin Gulaboski et al.
Cell calcium, 43(6), 615-621 (2007-11-16)
Fura-2 is one of the most commonly used fluorescent dyes to analyze the cytosolic Ca(2+) concentration ([Ca(2+)](i)) of living cells. Fura-2-dependent measurements of [Ca(2+)](i) are susceptible to changes of pH, reactive oxygen species concentration and membrane potential. Fura-2 is often
M Poenie et al.
Nature, 315(6015), 147-149 (1985-05-09)
Although the regulation of events in the cell division cycle by calcium or other cations has been the subject of much interest and speculation, experimental studies have been hampered by the difficulty of measuring submicromolar intracellular free calcium concentrations ([Ca2+]i)
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