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一般描述
IgG 是糖蛋白抗体,可调节几种免疫应答。针对靶蛋白的兔 IgG 常被用作各种研究应用的一抗。因此,与可检测底物偶联的二级抗兔 IgG 是分析靶蛋白的有用工具。已知山羊抗兔 IgG 与兔 IgG 相关。
免疫原
兔 IgG
应用
Atto荧光标记专门用于单分子检测等高灵敏度应用。 Atto标记的结构具有刚性,构型不会产生任何顺反异构变化。 因此这种标记偶联后的强度表现卓越,光谱转变却又极低。Atto 647N 山羊抗兔 IgG 已作为二抗(浓度为 5μg/ml),用于2% 甲醛固定细胞的免疫荧光。
成功使用该抗体的应用以及相关的同行评审论文如下所示。
免疫荧光分析(1篇论文)
免疫荧光分析(1篇论文)
外形
1 mg/mL 溶液(溶于 0.1 M 磷酸钠、0.1 MNaCl,pH 7.5,含 5 mM 叠氮化钠作为防腐剂)中提供了 1 mL 单位规格的 Atto 647 羊抗兔 IgG(全分子)。
分析说明
非结合染料≤总荧光的 5%
法律信息
本产品仅供研究使用。如果打算商业化,请联系知识产权持有者(德国ATTO-TEC GmbH公司)申请许可。
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves
法规信息
常规特殊物品
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免疫印迹(蛋白质印迹转印)是现代蛋白质组学研究中的常用技术。
Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.
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