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21877

5(6)-羧基荧光素

Name: 5(6)-羧基荧光素
Brand: SIGMA

BioReagent, suitable for fluorescence, ≥95% (HPLC)

别名:

5(6)-FAM, 5,6-CF

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关于此项目

经验公式（希尔记法）:

C₂₁H₁₂O₇

化学文摘社编号:

72088-94-9

分子量:

376.32

NACRES:

NA.32

PubChem Substance ID:

329752213

UNSPSC Code:

12171500

EC Number:

276-331-1

MDL number:

MFCD00151081

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属性

product line

BioReagent

Quality Level

200

assay

≥95% (HPLC)

form

powder

mp

275 °C (dec.) (lit.)

fluorescence

λ_ex 492 nm; λ_em 517 nm in 0.1 M Tris pH 8.0

suitability

suitable for fluorescence

SMILES string

OC(=O)c1ccc2c(c1)C(=O)OC23c4ccc(O)cc4Oc5cc(O)ccc35.OC(=O)c6ccc7C(=O)OC8(c9ccc(O)cc9Oc%10cc(O)ccc8%10)c7c6

InChI

1S/2C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21;22-11-2-5-14-17(8-11)27-18-9-12(23)3-6-15(18)21(14)16-7-10(19(24)25)1-4-13(16)20(26)28-21/h2*1-9,22-23H,(H,24,25)

InChI key

BPVHBBXCESDRKW-UHFFFAOYSA-N

说明

General description

5(6)-羧基荧光素，也称为5(6)-FAM，是一种荧光示踪剂，可作为5-羧基荧光素和6-羧基荧光素异构体的混合物。5(6)-羧基荧光素的荧光是由它的杂蒽部分决定的。 5(6)-羧基荧光素有两个特性:

它有两个波长的最大吸光度（465 和 490nm）。
它的荧光发射在 515nm处（最大）随 pH 值的变化而增大，生理范围为 6-7.4。

Application

5(6)-羧基荧光素在体内用于肿瘤细胞和正常细胞的表征。它标记通过n -羟基琥珀酰亚胺（NHS）酯介导衍生化衍生的蛋白质。 5(6)-羧基荧光素适用于检测细胞活力，同时开发体外驱虫药试验。

Other Notes

细胞内pH探针

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存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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