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Merck
CN

19899

Sigma-Aldrich

CHAPS 100 mM 溶液

别名:

CHAPS 溶液

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About This Item

经验公式(希尔记法):
C32H58N2O7S
CAS号:
分子量:
614.88
MDL编号:
UNSPSC代码:
12161902
PubChem化学物质编号:
NACRES:
NA.25

描述

zwitterionic

质量水平

表单

liquid

分子量

614.88 g/mol

浓度

100 mM in H2O

技术

protein quantification: suitable

CMC

8 mM

转变温度

cloud point >100 °C

密度

1.01 g/mL at 20 °C

储存温度

2-8°C

SMILES字符串

C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS([O-])(=O)=O)[C@H]1CC[C@H]2[C@@H]3[C@H](O)CC4C[C@H](O)CC[C@]4(C)[C@H]3C[C@H](O)[C@]12C

InChI

1S/C32H58N2O7S/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41)/t21-,22?,23-,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1

InChI key

UMCMPZBLKLEWAF-RFCNGIAKSA-N

一般描述

3-[(3-胆固醇氨丙基)二甲基氨基]-1-丙磺酸(CHAPS)是一种生物相容性表面活性剂。它是胆酸的衍生物,包含疏水的胆固醇样骨架,具有三个羟基和一个两性离子脂肪族尾。

应用

CHAPS 100 mM 溶液可用作裂解缓冲液的成分,用于提取山羊子宫内膜上皮细胞(EEC)和肝组织的蛋白质。

生化/生理作用

3-[(3-胆固醇氨丙基)二甲基氨基]-1-丙磺酸(CHAPS)具有硫代甜菜碱型表面活性剂和胆汁盐的特性。它参与蛋白质组学分析用蛋白质的提取过程,并使膜蛋白重建进入脂质囊泡内部(蛋白脂质体)。CHAPS被广泛应用于生物化学领域膜蛋白和受体的分离和纯化。由于其生物相容性、无电荷特性、非变性和非聚集特性,它可用于药物和医学领域。

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves


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