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生物来源
human mouth
质量水平
描述
Human squamous cell carcinoma
生长模式
Adherent
核型
Not specified
形态学
Polygonal
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
一般描述
The TR146 cells are appropriate for drug transport studies and they mimic normal human buccal epithelium. It serves as an oral mucosa model for barrier property studies with various molecules including tight junction proteins, aquaporins and epithelial-mesenchymal transition (EMT) markers.
细胞系来源
Human squamous cell carcinoma
细胞系描述
TR146 was derived from the neck node of a 67 year-old female (primary tumour was sited in buccal mucosa). The patient had had previous radiotherapy (6,000 rads) and neck dissection. TR146 is tumourigenic in female (nu/nu) mice leading to the production of rapidly progressing tumours. Histology: well-differentiated keratinizing squamous cell carcinoma.
应用
TR146 cell line human has been used:
- in cytotoxicity studies with an antibacterial compound, metronidazole
- in viability and metabolic studies post blue laser light exposure,
- as an oral epithelium-resembling cell line model for in vitro C. albicans infection studies
- in barrier function studies using transepithelial electrical resistance (TEER)
- for the transport studies with C-reactive protein (CRP)
DNA图谱分析
STR-PCR Data: Amelogenin: X
CSF1PO: 11,13
D13S317: 11,14
D16S539: 13
D5S818: 10,11
D7S820: 10,12
THO1: 9
TPOX: 8,9
vWA: 15,18
CSF1PO: 11,13
D13S317: 11,14
D16S539: 13
D5S818: 10,11
D7S820: 10,12
THO1: 9
TPOX: 8,9
vWA: 15,18
培养基
HAMS F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)
传代培养常规
Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at approximately 3 x 104 cells/cm2 using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. Saturation density of 5 x 104 cells/cm2.
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
免责声明
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
Correction.
Tissue barriers, 8(2), 1784644-1784644 (2020-07-03)
Drug development and industrial pharmacy, 25(4), 463-470 (1999-04-09)
Parameters that influence the precipitation of the beta-cyclodextrin (beta-CD) inclusion complexes of the antimycotics miconazole and econazole were investigated. The mechanistic reason for the superior antimycotic activity of the miconazole inclusion complex was studied. The toxicity of the complex was
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, 16(3), 151-157 (2002-07-20)
The present study was conducted to investigate and compare the effect of pH and drug concentration on nicotine permeability across the TR146 cell culture model and porcine buccal mucosa in vitro. As a further characterization of the TR146 cell culture
Methods in molecular biology (Clifton, N.J.), 2260, 133-143 (2021-01-07)
Microbial interactions with epithelial barriers are important steps preceding disease. Infections with Candida albicans are no exception. This opportunistic fungus, commonly harmlessly residing in close proximity to human epithelia, can shift to a more pathogenic form, can invade tissues, and
Archives of oral biology, 61, 149-155 (2015-11-19)
The mucosal pellicle is a thin layer of salivary proteins, mostly MUC5B mucins, anchored to epithelial oral cells. This pellicle is involved in protection of oral mucosae against abrasion, pathogenic microorganisms or chemical xenobiotics. The present study aimed at studying
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