N2125
萘酚 AS-BI 磷酸盐
≥98% purity (HPLC), powder
别名:
7-溴-3-羟基-2-萘-o-茴香醚磷酸盐
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关于此项目
经验公式（希尔记法）:
C18H15BrNO6P
化学文摘社编号:
分子量:
452.19
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
EC Number:
217-645-0
MDL number:
Beilstein/REAXYS Number:
6668526
产品名称
萘酚 AS-BI 磷酸盐, Technical grade, ≥98% (HPLC)
InChI key
HUXIAXQSTATULQ-UHFFFAOYSA-N
InChI
1S/C18H15BrNO6P/c1-25-16-5-3-2-4-15(16)20-18(21)14-9-12-8-13(19)7-6-11(12)10-17(14)26-27(22,23)24/h2-10H,1H3,(H,20,21)(H2,22,23,24)
SMILES string
COc1ccccc1NC(=O)c2cc3cc(Br)ccc3cc2OP(O)(O)=O
assay
≥98% (HPLC)
form
powder
technique(s)
microbe id | staining: suitable
color
white to light yellow
solubility
methanol: 50 mg/mL
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
−20°C
Quality Level
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Application
萘酚AS-BI磷酸可用于：
- 抗酒石酸酸性磷酸酶（TRAP）染色，以检测破骨细胞活性
- Tris-HCl染色细胞的组成部分，以测定碱性磷酸酶（ALP）的活性
- 进行碱性磷酸酶反应
酸性和碱性磷酸酶组织化学显示的底物。 酸性和碱性磷酸酶荧光测定法的底物。石蜡包埋组织中酸性磷酸酶的证明。单个活细胞中酸性磷酸酶的细胞荧光测定法。
Biochem/physiol Actions
色酚AS-BI可用作测定碱性和酸性磷酸酶的底物。也可用于测定单细胞酸性磷酸酶的活性。
General description
色酚AS-BI（也称为7-溴代-3- 羟基-2-萘酚-o-茴香胺）是一种取代萘酚。
酸性和碱性磷酸酶组织化学显示的底物。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
The presence of a low molecular weight acid phosphatase in liver tissue that cannot be demonstrated with the histochemical substrate naphthol AS-BI phosphate.
A E Meijer et al.
Histochemistry, 67(1), 23-29 (1980-01-01)
W M Frederiks et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 35(2), 175-180 (1987-02-01)
Acid phosphatase activity has been demonstrated in rat liver with the semipermeable membrane technique using naphthol AS-BI phosphate as substrate and hexazotized pararosaniline (HPRA) as simultaneous coupling agent. With this method the final reaction product (FRP) appeared in rat liver
Z Szegedi et al.
Pathology oncology research : POR, 4(3), 217-223 (1998-10-08)
The aim of the study was to work out a technique for the detection of acid phosphatase enzyme activity by confocal laser-scanning microscope using the histochemical acid phosphatase detection method (after Barka and Anderson 1962, modified by Bowen and Lewis
Quantitative histochemical investigations of semipermeable membrane techniques for the assay of acid phosphatase in skeletal muscle. I. Meijer's method.
P J Stoward et al.
Histochemistry, 71(3), 405-431 (1981-01-01)
Naphthol-ASBI phosphate as a preferred substrate for tartrate-resistant acid phosphatase isoform 5b.
A J Janckila et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 16(4), 788-793 (2001-04-24)
Tartrate-resistant acid phosphatase (TRAP) isoform 5b is a potential serum marker for osteoclastic activity. Biochemical assays for serum TRAP activity with para-nitrophenylphosphate (pNPP) have low specificity for bone because of hydrolysis by unrelated nontype 5 TRAPs of blood cells and
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