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Merck
CN

I5502

Sigma-Aldrich

异丙基β-D-1-硫代吡喃半乳糖苷

≥99% (TLC)

别名:

IPTG, 异丙基β-D-硫代半乳糖苷

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About This Item

经验公式(希尔记法):
C9H18O5S
CAS号:
分子量:
238.30
Beilstein:
4631
EC 号:
MDL编号:
UNSPSC代码:
12352201
PubChem化学物质编号:
NACRES:
NA.31

描述

non-ionic

质量水平

检测方案

≥99% (TLC)

形式

powder

技术

thin layer chromatography (TLC): suitable

杂质

10.0-22.0% Dioxane

颜色

white to off-white

mp

120 -122  °C ((248 - 252 °F ))

溶解性

water: 50 mg/mL, clear, colorless

储存温度

2-8°C

SMILES字符串

CC(C)S[C@@H]1OC(CO)[C@H](O)C(O)[C@H]1O

InChI

1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1

InChI key

BPHPUYQFMNQIOC-NXRLNHOXSA-N

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应用

IPTG通常用于需要诱导β-半乳糖苷酶活性的克隆程序。它与 X-Gal 或 Bluo-Gal 一起用于重组细菌菌落的蓝白斑筛选,其诱导 lac 操纵子在大肠杆菌中的表达。IPTG 通过与 lacI 阻遏物结合,并改变其构象起作用,其阻止 β-半乳糖苷酶编码基因 lacZ 的阻遏作用。

其他说明

非代谢性半乳糖类似物。
为了全面了解我们针对客户研究提供的各种单糖产品,建议您访问我们的碳水化合物分类页面。

重悬

将 IPTG 溶于水,然后经无菌过滤,配制成储存液 (0.1M)。指示板中 IPTG 最终浓度应为 0.2mM。

警示用语:

WarningDanger

危险分类

Carc. 1B - Eye Irrit. 2

补充剂危害

储存分类代码

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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