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Merck
CN

E8751

溴化乙锭

~95% (HPLC)

别名:

3,8-二氨基-5-乙基-6-苯基菲啶溴化物, EtBr, 胡米溴铵

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关于此项目

经验公式（希尔记法）:
C21H20BrN3
化学文摘社编号:
1239-45-8
分子量:
394.31
UNSPSC Code:
41105322
NACRES:
NB.22
PubChem Substance ID:
24894683
EC Number:
214-984-6
Beilstein/REAXYS Number:
3642536
MDL number:
MFCD00011724

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产品名称

溴化乙锭, ~95% (HPLC)

InChI

1S/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14;/h3-13,23H,2,22H2,1H3;1H

InChI key

ZMMJGEGLRURXTF-UHFFFAOYSA-N

SMILES string

[Br-].CC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14

assay

~95% (HPLC)

mp

260-262 °C (dec.) (lit.)

Quality Level

200

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Application

溴化乙锭 (EtBr) 是 PAGE 或琼脂糖凝胶电泳最常用的核酸染色剂。EtBr 的荧光在与双链 RNA 结合后可增加 21 倍，在与双链 DNA 结合后增加 25 倍，因此在低染色浓度（10μg/ml）下无需本底脱色。溴化乙锭已用于大量核酸荧光测定。 它已被证明可与单链 DNA（虽然没有那么强）和三链 DNA 结合。由于它能与 DNA 结合，EtBr 是 DNA 聚合酶的抑制剂。

插入双链 DNA 和 RNA 的移码诱变剂。

Biochem/physiol Actions

溴化乙啶可插入双链 DNA 和 RNA 中，并作为移码诱变剂。它还可与吖啶橙结合用于区分存活的、凋亡的和坏死的细胞。

Preparation Note

对于在电泳后进行凝胶染色，应先用水将储存液样品稀释到 0.5μg/ml，再将凝胶孵育 15-30 分钟。通常无需脱色，但是如果必须降低背景色，则可在水中脱色 15 分钟。然后可在紫外灯箱（254nm 波长）中检测 DNA 条带。也可将溴化乙啶加到凝胶和电泳缓冲液（终浓度 0.5μg/ml）中，并在电泳后立即显影。

pictograms

Skull and crossbonesHealth hazard
GHS06,GHS08

signalword

Danger

Hazard Classifications

Acute Tox. 1 Inhalation - Acute Tox. 4 Oral - Muta. 2

存储类别

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SHBT1421
SHBS8107
SHBS3878
SHBR4830
SHBQ7920

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the best working concentration of Product E8751, Ethidium bromide?

    For staining a gel after electrophoresis, dilute a sample of the stock solution to 0.5 μg/mL with water and incubate the gel for 15-30 min.  Ethidium bromide can also be incorporated into the gel and running buffer at 0.5 μg/mL, and visualized immediately after electrophoresis.

  5. How can I make a stock solution of Product E8751, Ethidium bromide?

    Ethidium bromide is soluble in water at 100 mg/mL. Per Crary, B. and Boyrsenko, M., Biotechniques, 4, 98-101 (1986), stock solutions of ethidium bromide in water or PBS are stable for at least two years at room temperature if protected from light.

  6. How can I dispose of solutions containing Product E8751, Ethidium bromide?

    Ethidium bromide is mutagenic, and ultimately has to be destroyed by incineration. It can be removed from buffer solutions by several methods to reduce the volume of waste. See the product sheet for more details.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Ethidium Bromide

Ethidium bromide is a well-known and widely used fluorescent dye in biotechnology research.

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