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01494

Sigma-Aldrich

Nancy-520

greener alternative

suitable for RT-PCR, ≥95.0% (HPCE)

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About This Item

EC 号:
200-664-3
UNSPSC代码:
12171500
NACRES:
NA.32

质量水平

100

方案

≥95.0% (HPCE)

表单

liquid

环保替代产品特性

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

溶解性

DMSO: soluble

荧光

λex 520 nm; λem 554 nm in TE buffer; DNA

环保替代产品分类

Aligned,

储存温度

2-8°C

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相关类别

一般描述

我们致力于为您带来更加绿色的替代产品，这些产品遵守一项或多项绿色化学12项原则。该产品经过专门设计，提高了化学品的安全性

应用

Nancy-520 是琼脂糖电泳凝胶上 dsDNA 的荧光染料，比溴化乙锭具有更高的灵敏度，并且染色程序简便、快速和稳健。其作为5000x 储备液（500μl）提供—这对于 50 个琼脂糖凝胶就足够了。检测限为 0.5 ng/dsDNA带。
此外，Nancy-520 可用于测定溶液中 dsDNA 浓度，线性范围在0-2μg/ml DNA。 Nancy 520

Nancy-520 已成功用于 PCR（实时 PCR）应用。
Nancy 520

溴化乙锭更环保的替代品。

包装

无底玻璃瓶。内含物装在插入式熔锥内。

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说明
价格

储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(°F)

188.6 °F - closed cup - Solvent

闪点(°C)

87 °C - closed cup - Solvent

个人防护装备

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

从最新的版本中选择一种：

分析证书(COA)

Lot/Batch Number
BCCK2837
BCCK0120
BCCG0702
BCCC9564
BCBX4189

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  1. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  2. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  3. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  4. What is the mutagenic potential of Product 01494, Nancy-520, compared to SYBR Green I and ethidium bromide?

    The Ames test (II) has confirmed that Product 01494, Nancy-520, is less mutagenic than SYBR green I and ethidium bromide.

  5. What is the sensitivity of Product 01494, Nancy-520, compared to SYBR Green I and ethidium bromide?

    Product 01494, Nancy-520, shows a higher sensitivity than ethidium bromide and a slightly higher sensitivity to SYBR Green I.

  6. Does Product 01494, Nancy-520, require the same safety handling that is required for Ethidium bromide?

    No, the handling of Product 01494, Nancy-520, does not require the strict safety instructions that are needed for Ethidium bromide. Normal latex gloved may be used. No specific "Nitrile" gloves are required. No particularly equipped fume hood for Nancy-520 is required. Other work can be done in the same fume hood, where Nancy-520 is used.

  7. Can Product 01494, Nancy-520 staining solution, be reused?

    Re-use of Product 01494, Nancy-520 staining solution, is not recommended.

  8. Can Nancy-520 be detected when using the Odyssey® Fc imager?

    According to an application note published by LI-COR "Imaging Nucleic Acid Gels on the Odyssey® Fc Imager", published in 2011, Nancy-520 was successfully tested in the Fc imager, using the 600 channel.®Odyssey is a registered Trademark of LI-COR, Inc. The trademark belongs to their respective owners. The Odyssey® Fc Imaging system is covered by U.S. patents, foreign equivalents, and patents pending.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Park, D.J.
PCR Protocols (2011)
Rodriguez-Lazaro, D.
Real-Time PCR in Food Science: Current Technology and Applications (2013)
Saunders, N.A. and Lee, M.A.
Real-Time PCR: Advanced Technologies and Applications (2013)
van Pelt-Verkuil, E., van Belkum, A. and Hays, J.P
Principles and Technical Aspects of PCR Amplification (2008)
Filion, M.
Quantitative Real-time PCR in Applied Microbiology (2012)
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商品

Quantitative PCR Basics

Real-time polymerase chain reaction allows researchers to estimate the quantity of starting material in a sample. It has a much wider dynamic range of analysis than conventional PCR

定量PCR基础知识

实时聚合酶链式反应可用于计算样本的起始材料量。它相比常规PCR具有更大的检测动态范围。

相关内容

DNA Detection

Nancy-520 for DNA Detection and Quantitation

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