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Merck
CN
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文件

D5545

Sigma-Aldrich

DL-二硫苏糖醇

BioXtra, ≥99.0% (titration)

别名:

1,4-二巯基苏糖醇, DTT, 克莱兰氏试剂

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About This Item

线性分子式:
HSCH2CH(OH)CH(OH)CH2SH
CAS号:
3483-12-3
分子量:
154.25
Beilstein:
1719757
EC 号:
222-468-7
MDL编号:
MFCD00004877
UNSPSC代码:
12352201
PubChem化学物质编号:
24893941
NACRES:
NA.21

产品线

BioXtra

质量水平

200

检测方案

≥99.0% (titration)

形式

powder

反应适用性

reagent type: reductant

杂质

Insoluble matter, passes filter test
≤0.3% 4,5-dihydroxy-1,2-dithiane

pH值（酸碱度）

4.0-6.0 (20-25 °C, 0.1 m in H2O)

mp

41-44 °C (lit.)

溶解性

H2O: 0.1 M, clear, colorless

痕量阴离子

sulfate (SO42-): ≤0.005%

痕量阳离子

Al: ≤0.0005%
As: ≤0.0001%
Ba: ≤0.0005%
Bi: ≤0.0005%
Ca: ≤0.001%
Cd: ≤0.0005%
Co: ≤0.0005%
Cr: ≤0.0005%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Li: ≤0.0005%
Mg: ≤0.0005%
Mn: ≤0.0005%
Mo: ≤0.0005%
Na: ≤0.1%
Ni: ≤0.0005%
Pb: ≤0.0005%
Sr: ≤0.0005%
Zn: ≤0.0005%

吸光度

≤0.100 at 280 in H2O at 0.1 M
≤0.400 at 260 in H2O at 0.1 M

储存温度

2-8°C

SMILES字符串

O[C@H](CS)[C@H](O)CS

InChI

1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1

InChI key

VHJLVAABSRFDPM-QWWZWVQMSA-N

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相关类别

应用

用于将 −SH 基保持在还原态的绝佳试剂；定量还原二硫化物。在 SDS-PAGE 前，DTT 在样品缓冲液中可有效还原蛋白二硫键。DTT 还可用于还原交联剂 N,N′-双(丙烯酰)胱胺的二硫键桥以分解聚丙烯酰胺凝胶基质。DTT 的刺激性和毒性都低于 2-巯基乙醇。通常，使用 DTT (100mM) 所需的浓度是使用 2-巯基乙醇 (5% v/v, 700mM) 的浓度的七分之一。

象形图

CorrosionExclamation mark
GHS05,GHS07

警示用语：

Danger

危险声明

H302,H315,H318

危险分类

Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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分析证书(COA)

Lot/Batch Number

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Alessio Grimaldi et al.
Communications biology, 3(1), 85-85 (2020-02-27)
Here, we developed an unbiased, functional target-discovery platform to identify immunogenic proteins from primary non-small cell lung cancer (NSCLC) cells that had been induced to apoptosis by cisplatin (CDDP) treatment in vitro, as compared with their live counterparts. Among the
Carl M Sellgren et al.
Nature neuroscience, 22(3), 374-385 (2019-02-06)
Synapse density is reduced in postmortem cortical tissue from schizophrenia patients, which is suggestive of increased synapse elimination. Using a reprogrammed in vitro model of microglia-mediated synapse engulfment, we demonstrate increased synapse elimination in patient-derived neural cultures and isolated synaptosomes.
Kuang Shen et al.
Cell, 179(6), 1319-1329 (2019-11-11)
mTORC1 controls anabolic and catabolic processes in response to nutrients through the Rag GTPase heterodimer, which is regulated by multiple upstream protein complexes. One such regulator, FLCN-FNIP2, is a GTPase activating protein (GAP) for RagC/D, but despite its important role
Xueqian Liu et al.
The Journal of biological chemistry, 288(10), 7230-7240 (2013-01-25)
In Pichia pastoris, the peroxisomal targeting signal 2 (PTS2)-dependent peroxisomal matrix protein import pathway requires the receptor, Pex7, and its co-receptor Pex20. A conserved lysine (Lys(19)) near the N terminus of Pex20 is required for its polyubiquitination and proteasomal degradation
Hanayo Ueoka-Nakanishi et al.
The FEBS journal, 280(14), 3220-3231 (2013-04-26)
Thioredoxin (Trx) is a key player in redox homeostasis in various cells, modulating the functions of target proteins by catalyzing a thiol-disulfide exchange reaction. Target proteins of cytosolic Trx-h of higher plants were studied, particularly in the plasma membrane, because
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