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Merck
CN

D3435

Millipore

Dey-Engley 中和肉汤

suitable for microbiology, NutriSelect® Plus

别名:

D/E中和肉汤

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About This Item

UNSPSC代码:
41171607
NACRES:
NA.85

无菌性

non-sterile

质量水平

形式

powder

保质期

limited shelf life, expiry date on the label

制造商/商品名称

NutriSelect® Plus

最终pH

7.6±0.2 (25 °C)

应用

environmental
food and beverages
pharmaceutical
sterility testing
surface monitoring

microbiology

储存温度

2-8°C

适用性

nonselective for Bacillus spp.
nonselective for Escherichia coli
nonselective for Pseudomonas spp.
nonselective for Salmonella spp.
nonselective for Staphylococcus spp.
nonselective for bacteria (General Media)
nonselective for coliforms

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一般描述

Dey-Engley中和肉汤用于消毒剂测试,其中防腐剂和消毒剂的中和对于测定杀菌活性很重要。肉汤由提供氮和碳源的色氨酸、长链氨基酸、维生素和其他必需营养素组成。葡萄糖是一种能量来源。酵母提取物也是维生素B复合物的丰富来源。亚硫酸氢钠中和醛;巯基乙酸钠中和汞;硫代硫酸钠中和碘和氯;卵磷脂中和季铵化合物;聚山梨酸酯80,一种非离子表面活性剂,中和取代酚。溴甲酚紫色是葡萄糖利用的指标。发酵葡萄糖的微生物会把培养基从紫色变成黄色。假单胞菌属的生长不会发酵葡萄糖,可通过在肉汤表面形成的膜来检测。

应用

Dey-Engley中和培养基用于预湿润海绵拭子样本中和消毒剂对微生物生长的残留作用。Dey-Engley中和肉汤用于消毒剂测试,其中利用防腐剂和消毒剂的中和来测定杀菌活性。此外,还用于重新悬浮Vermamoeba vermiformis(哈氏虫属)囊肿,以中和过乙酸(PAA)。
D-E 中和肉汤在消毒实验中用于中和防腐剂和消毒剂,这对于检测细菌活性非常重要。

生化/生理作用

Dey-Engley中和培养基用于中和广泛的防腐剂和消毒剂,包括季铵化合物、酚类、碘类、氯、汞、甲醛和戊二醛等。巯基乙酸钠、硫代硫酸钠、亚硫酸氢钠、大豆卵磷脂和聚山梨酯80均用作中和成分。从紫色到黄色的颜色变化表示生长。

组分

成分 (g/L)
酶水解酪蛋白 5.00
酵母提取物 2.50
右旋糖 10.00
硫代硫酸钠 6.00
巯基乙酸钠 1.00
亚硫酸氢钠 2.50
卵磷脂 7.00
聚山梨酸酯 80 5.00
溴甲酚紫 0.02

制备说明

将39克Dey-Engley中和培养基悬浮于1000毫升蒸馏水中。煮沸至培养基完全溶解。使用15磅压力(121°C)进行高压灭菌的方法消毒15分钟。

附注

我们现提供两种类型的培养基:GranuCult®优质颗粒状培养基和NutriSelect®高性价比粉末状培养基,可满足用户不同层次的需求。
商标后的品级称号basic(基础)、plus(加强)或prime(特级)代表产品的不同质控水平,从基础的质控到标准质控加强版到全面合规的特级质控水平。

法律信息

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Skin Sens. 1

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Faceshields, Gloves

法规信息

动植物来源培养基

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分析证书(COA)

Lot/Batch Number

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D Joseph Sexton et al.
Infection control and hospital epidemiology, 41(10), 1219-1221 (2020-07-01)
We tested 9 disinfectants against Candida auris using the quantitative disk carrier method EPA-MB-35-00: 5 products with hydrogen peroxide or alcohol-based chemistries were effective and 4 quaternary ammonium compound-based products were not. This work supported a FIFRA Section 18 emergency
Sensitivity of Vermamoeba (Hartmannella) vermiformis cysts to conventional disinfectants and protease
Fouque E, et al.
Journal of Water and Health, 13(2), 302-310 (2015)
H Maertens et al.
BMC veterinary research, 16(1), 140-140 (2020-05-20)
Disinfectants are frequently used in animal production to reduce or eliminate the load of infectious agents and parasites in buildings and equipment associated with the housing or transportation of animals. There are growing concerns that the use of disinfectants would
Comparison of competitive exclusion with classical cleaning and disinfection on bacterial load in pig nursery units
Luyckx K, et al.
BMC Veterinary Research, 12(1), 189-189 (2016)
H Maertens et al.
Poultry science, 98(7), 2972-2976 (2019-04-18)
Resistance to antibiotics threatens to become a worldwide health problem. An important attributing phenomenon in this context is that pathogens can acquire antibiotic resistance genes through conjugative transfer of plasmids. To prevent bacterial infections in agricultural settings, the use of

商品

一篇关于假单胞菌属的检测、鉴定、鉴别和培养的文章。

An article concerning the detection, identification, differentiation, and cultivation of Pseudomonas species.

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