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Merck
CN

D0531

Sigma-Aldrich

5-([4,6-二氯三嗪-2-基]氨基)荧光素 盐酸盐

≥90% purity (HPLC), powder

别名:

DTAF

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About This Item

经验公式(希尔记法):
C23H12Cl2N4O5 · HCl
CAS号:
分子量:
531.73
MDL编号:
UNSPSC代码:
12171500
PubChem化学物质编号:
NACRES:
NA.47

product name

5-([4,6-二氯三嗪-2-基]氨基)荧光素 盐酸盐, ≥90% (HPLC)

质量水平

检测方案

≥90% (HPLC)

形式

powder

颜色

yellow to orange

溶解性

methanol: 20 mg/mL, clear to slightly hazy, yellow to orange

荧光

λex 495 nm; λem 516 nm

应用

diagnostic assay manufacturing
hematology
histology

储存温度

2-8°C

SMILES字符串

Cl.Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(Nc6nc(Cl)nc(Cl)n6)ccc45)c1

InChI

1S/C23H12Cl2N4O5.ClH/c24-21-27-22(25)29-23(28-21)26-10-1-4-13(16(7-10)20(32)33)19-14-5-2-11(30)8-17(14)34-18-9-12(31)3-6-15(18)19;/h1-9,30H,(H,32,33)(H,26,27,28,29);1H

InChI key

CEKSHCAIHGNWBK-UHFFFAOYSA-N

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应用

5-([4,6-二氯三嗪-2-基]氨基)荧光素盐酸盐已用于标记酵母细胞,以研究酵母细胞对欧洲鲈鱼(Dicentrarchus labrax) 幼鱼和豹纹喙鲈(Mycteroperca rosacea)消化道的粘附。此外,它还被用于标记细菌(弧菌属
)。5-([4,6-二氯三嗪-2-基]氨基)荧光素盐酸盐已被用来共价标记黄原胶(XG)以研究其明胶行为模式。
一种荧光素衍生物,可在中性 pH 下与蛋白质的氨基和 N 末端氨基酸反应。

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


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Michael S Reid et al.
Carbohydrate polymers, 250, 116943-116943 (2020-10-15)
Fluorescently labeled cellulose nanofibrils (CNFs) were used to evaluate CNF leaching from paper according to standard safety assays for food contact materials. Enzymatically pretreated pulp was first labeled with 5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein hydrochloride (DTAF), followed by homogenization to produce fluorescent CNFs of
Adrián Pérez-Ramos et al.
Frontiers in microbiology, 9, 1684-1684 (2018-08-10)
Lactic acid bacteria synthesize exopolysaccharides (EPS), which could benefit the host's health as immunomodulators. Furthermore, EPS could protect bacteria against gastrointestinal stress, favoring gut colonization, thus protecting the host against pathogenic infections. Pediococcus parvulus 2.6, produces a 2-substituted (1,3)-β-D-glucan and
Assessment of fluorescent-labeled bacteria for evaluation of in vivo uptake of bacteria (Vibrio spp.) by crustacean larvae.
Soto-Rodriguez SA, et al.
Journal of Microbiological Methods, 52, 101-114 (2003)
Synergistic gelation of gelatin B with xanthan gum
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Wang CS, et al.
Food Hydrocolloids, 79, 462-472 (2018)

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