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Merck
CN

C4916

Sigma-Aldrich

5-羧荧光素二乙酸酯

~95% purity (HPLC), powder

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别名:
5-CFDA, 5-羧基-二- O -乙酰荧光素
经验公式(希尔记法):
C25H16O9
CAS号:
分子量:
460.39
Beilstein:
68265
MDL编号:
UNSPSC代码:
12171500
PubChem化学物质编号:
NACRES:
NA.47

检测方案

~95% (HPLC)

质量水平

形式

powder

溶解性

chloroform/ethanol (1:1): 9.80-10.20 mg/mL, clear

应用

diagnostic assay manufacturing
hematology
histology

储存温度

−20°C

SMILES字符串

CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5cc(ccc45)C(O)=O)c1

InChI

1S/C25H16O9/c1-12(26)31-15-4-7-19-21(10-15)33-22-11-16(32-13(2)27)5-8-20(22)25(19)18-6-3-14(23(28)29)9-17(18)24(30)34-25/h3-11H,1-2H3,(H,28,29)

InChI key

WPUZGNPQMIWOHE-UHFFFAOYSA-N

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应用

5-羧基荧光素二乙酸酯已用于细菌的染色,以研究细菌对黏蛋白的粘附。

生化/生理作用

细胞的染色需要 5-羧基荧光素二乙酸酯来区分活细胞和死细胞。有助于监测细胞膜的完整性和完整性。5-羧基荧光素二乙酸酯也用于测量细胞内 pH 值。5-羧基荧光素二乙酸酯是一种非荧光分子,被细胞内酯酶裂解,从而产生绿色荧光产物羧基荧光素。在活细胞中,细胞膜对荧光分子是不可渗透的。

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges


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