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文件

安全信息

B6529

Sigma-Aldrich

亮蓝R染色液

ethanol solution

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别名:
酸性蓝83, Coomassie 亮蓝R, 亮吲哚蓝蛋白 6B, 酸性蓝 83
经验公式（希尔记法）:
C45H44N3NaO7S2
CAS号:
6104-59-2
分子量:
825.97
颜色索引号:
42660
Beilstein:
5718025
MDL编号:
MFCD00041762
UNSPSC代码:
12171500
PubChem化学物质编号:
24891914
NACRES:
NA.47

形式

liquid

质量水平

200

颜色

dark blue

适用性

suitable for (for immunoelectrophoresis protein staining)

应用

diagnostic assay manufacturing
hematology
histology

储存温度

room temp

SMILES字符串

[Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5\C=C/C(C=C5)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1

InChI

1S/C45H45N3O7S2.Na/c1-4-47(31-33-9-7-11-43(29-33)56(49,50)51)40-23-15-36(16-24-40)45(35-13-19-38(20-14-35)46-39-21-27-42(28-22-39)55-6-3)37-17-25-41(26-18-37)48(5-2)32-34-10-8-12-44(30-34)57(52,53)54;/h7-30H,4-6,31-32H2,1-3H3,(H2,49,50,51,52,53,54);/q;+1/p-1

InChI key

NKLPQNGYXWVELD-UHFFFAOYSA-M

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相关类别

应用

亮蓝R染色溶液专门设计用于在免疫电泳和Ouchterlony凝胶之后对琼脂糖凝胶中的蛋白质进行染色。污渍含有乙醇和乙酸，因此在染色前无需固定凝胶。首先用水和盐水洗涤免疫电泳或Ouchterlony凝胶，以去除未沉淀的蛋白质，然后干燥。然后将凝胶浸入染色溶液中30分钟，并用10%乙酸脱色。
电泳后检测蛋白条带。

组分

0.5% (w/v) 亮蓝 R、45% (v/v) 乙醇和 10% (v/v) 乙酸。

分析说明

在免疫电泳后测试琼脂糖凝胶的适用性。

法律信息

象形图

FlameExclamation mark
GHS02,GHS07

警示用语：

Warning

危险声明

H226,H315,H319

危险分类

Eye Irrit. 2 - Flam. Liq. 3 - Skin Irrit. 2

WGK

WGK 2

闪点(°F)

81.0 °F

闪点(°C)

27.2 °C

个人防护装备

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

法规信息

危险化学品

分析证书(COA)

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Wei Zhang et al.
International journal of oncology, 54(5), 1719-1733 (2019-03-14)
Ovarian cancer remains the most lethal type of cancer among all gynecological malignancies. The majority of patients are diagnosed with ovarian cancer at the late stages of the disease. Therefore, there exists an imperative need for the development of early
Recovery of single-band proteins from polyacrylamide gels by using electrophoresis in solutions with different ionic strengths and specific weights.
Ding-Gan Liu
Analytical biochemistry, 339(2), 351-352 (2005-03-31)
G Sreeramulu et al.
Electrophoresis, 16(3), 362-365 (1995-03-01)
A novel method for destaining of polyacrylamide gels, stained with Coomassie Brilliant Blue R-250, is described, based on the use of 0.5 M NaCl in water as the destainer, requiring only 2-3 h. Concentrated (> 2 M) or dilute (<
G Houen et al.
Electrophoresis, 18(5), 701-705 (1997-05-01)
A method for staining proteins on polyvinylidene difluoride membranes without using organic solvent is described. The method uses preblocking of the membrane with either Tween 20 or polyethylene glycol followed by staining with 0.01% Coomassie Brilliant Blue. No destaining of
Melda Altikatoglu et al.
Artificial cells, blood substitutes, and immobilization biotechnology, 39(3), 185-190 (2010-12-02)
Horseradish peroxidase (EC 1.11.1.7) was chemically modified by periodate-activated dextran. The activities of free and modified enzyme against organic-aqueous interface and some chemicals were determined. Modified HRP remained fully active in the presence of organic solvent for 4 h. However
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