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Merck
CN

94635

革兰氏藏红 溶液

suitable for microscopy

别名:

藏红 T 溶液

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关于此项目

经验公式（希尔记法）:
C20H19ClN4
化学文摘社编号:
477-73-6
分子量:
350.84
UNSPSC Code:
12171500
NACRES:
MA.02
PubChem Substance ID:
329770368
MDL number:
MFCD00011759
Beilstein/REAXYS Number:
3924099

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产品名称

革兰氏藏红 溶液, suitable for microscopy

InChI

1S/C20H18N4.ClH/c1-12-8-17-19(10-15(12)21)24(14-6-4-3-5-7-14)20-11-16(22)13(2)9-18(20)23-17;/h3-11H,1-2H3,(H3,21,22);1H

SMILES string

[Cl-].Cc1cc2nc3cc(C)c(N)cc3[n+](-c4ccccc4)c2cc1N

InChI key

OARRHUQTFTUEOS-UHFFFAOYSA-N

form

liquid

shelf life

limited shelf life, expiry date on the label

composition

ethanol, 10%

technique(s)

microbe id | staining: suitable

color

red to very dark red

refractive index

n20/D 1.339

solubility

water: soluble at 20 °C

density

0.986 g/mL at 20 °C

εmax

4.0 at 532 nm in 50% ethanol

suitability

suitable for microscopy

antibiotic activity spectrum

Gram-negative bacteria
Gram-positive bacteria

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

100

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相关类别

Application

革兰氏番红溶液已用于以下应用：
  • 作为无毒结晶紫替代品，用于生物膜形成的定量
  • 开发透明质酸可视化方法
  • 研究如何通过2-氨基噻唑改善β-内酰胺对生物膜内嵌金黄色葡萄球菌的敏感性。

Biochem/physiol Actions

革兰氏染色可区分革兰氏阳性和革兰氏阴性细菌。细菌细胞壁的胞壁质结构是颜色亲和力的基础。首先用结晶紫给细菌染色，然后用碘溶液染色，形成染料-碘复合物。这种复合物在脱色后保留在革兰氏阳性菌细胞壁的多层粘肽结构中，形成蓝紫色。 革兰氏阴性细菌的细胞壁由单层粘肽结构组成，不会保留染料-碘复合物，可吸收番红复染液，从而呈现粉红色。

Features and Benefits

  • 可直接使用的 溶液。
  • 经过改进和设计，使染色可以在 染色细胞、染色架和自动染色系统中进行。

General description

革兰氏番红溶液，又称番红T或碱性红2，是一种即用的染色溶液，包含10%的乙醇番红。番红是一种碱性、阳离子、偶氮染料，含有一个平面的、二氨基吩嗪发色团，苯基取代基连接到一个环氮上。番红的三甲基阳离子形式是亲脂性的，而二甲基形式是弱亲水性的。它最常用于细菌学中的革兰氏染色复染，以区分革兰氏阳性菌和革兰氏阴性菌。

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Hazard Classifications

Flam. Liq. 3

存储类别

3 - Flammable liquids

wgk

WGK 2

flash_point_f

114.8 °F

flash_point_c

46 °C

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
BCCN4100
BCCM8024
BCCL8947
BCCK9963
BCCK2804

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