推荐产品
无菌性
sterile (Filtered and Aseptic Handled)
质量水平
形式
powder
组成
glucose, 20 mM
magnesium chloride, 10 mM
magnesium sulfate, 10 mM
potassium chloride, 2.5 mM
sodium chloride, 10 mM
tryptone, 20 g/L
yeast extract, 5 g/L
技术
microbiological culture: suitable
最终pH
7.0±0.2 (25 °C)
应用
food and beverages
life science and biopharma
microbiology
储存温度
2-8°C
适用性
nonselective for Escherichia coli
nonselective for coliforms
应用
SOC Broth is a sterile solution for the recovery of electroporated Escherichia coli cells.
制备说明
Immediately after electroporation add 1 ml sterile SOC Medium to the transformed cells and incubate for 30-60 minutes at 37°C with a moderate shaking .
其他说明
大肠杆菌质粒的转化
附注
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
法律信息
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves
法规信息
动植物来源培养基
Advanced Bacterial Genetics, Cold Spring Harbor (2007)
F.M. Ausubel et al.
Current Protocols in Molecular Biology (1987)
D Hanahan
Journal of molecular biology, 166(4), 557-580 (1983-06-05)
Factors that affect the probability of genetic transformation of Escherichia coli by plasmids have been evaluated. A set of conditions is described under which about one in every 400 plasmid molecules produces a transformed cell. These conditions include cell growth
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门