Agency
according to ISO 16654:2001
质量水平
无菌性
non-sterile
形式
powder
保质期
limited, expiry date on the label
组成
bile salts No. 3, 1.5 g/L
di-potassium hydrogen phosphate, 4.0 g/L
D(+)-glucose, 2.5 g/L
novobiocin
peptone from casein, 17.0 g/L
peptone from soymeal, 3.0 g/L
sodium chloride, 5.0 g/L
制造商/商品名称
NutriSelect® Plus
包装
pkg of -500 g
储存条件
(Dry place, tightly sealed containers)
技术
microbiological culture: suitable
颜色
faint brown
最终pH
7.3±0.2 (25 °C)
应用
agriculture
food and beverages
microbiology
pathogen testing
运输
wet ice
储存温度
2-8°C
适用性
selective for Escherichia coli
selective for coliforms
selective for enterovirulent E. coli (EEC)
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一般描述
mTSB Broth with Novobiocin is suitable for the selective enrichment of enterohemorrhagic Escherichia coli (EHEC) in foods intended for human and animal consumption. The peptone provides amino acids and other complex nitrogenous substances. Glucose is a fermentable carbohydrate. Sodium chloride delivers osmotic balance. Bile salts inhibit the growth of Gram-positive accompanying bacterial flora. Di-potassium hydrogen phosphate is the buffering agent to stabilize the pH.mTSB is made selective for E. coli O157:H7 by adding bile salts in the media along with Novobiocin supplementation.
应用
用于食品中肠出血性大肠杆菌 (EHEC) 的选择性增菌。根据 USDA-FSIS 方法用于分离和鉴别肉类中的大肠杆菌 0157:H7。
制备说明
Suspend 33 g in 1 litre of distilled water, autoclave at 121°C for 15 minutes.
附注
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
法律信息
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
动植物来源培养基
Isolation and molecular characterization of Salmonella enterica,
Escherichia coli O157:H7 and Shigella spp. from meat and dairy products
in Egypt
Escherichia coli O157:H7 and Shigella spp. from meat and dairy products
in Egypt
Ashraf M. Ahmed and Tadashi Shimamoto
International Journal of Food Microbiology, 57-62 (2014)
Optimization of enrichment and plating procedures for the recovery of Escherichia coli O111 and O26 from minced beef
T.M.G. Catarame
Journal of Applied Microbiology, 95(5) (2003)
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