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Merck
CN

70191

Mueller Hinton 琼脂

NutriSelect® Plus, according to FDA, NCCLS, powder, for bacteria, pack of 500 g, pack of 100 g, pack of 2.5 kg

别名:

Mh琼脂：抗生素敏感性测试介质；纸片扩散测试介质

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关于此项目

NACRES:
NA.74
UNSPSC Code:
41171607

主要文件

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产品名称

Mueller Hinton 琼脂, NutriSelect® Plus, according to FDA, NCCLS, powder, for bacteria, pack of 500 g, pack of 100 g, pack of 2.5 kg

agency

NCCLS

reg. compliance

according to FDA

sterility

non-sterile

product line

BioChemika

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 17.0 g/L
beef infusion solids, 2.0 g/L
casein hydrolysate, 17.5 g/L
starch, 1.5 g/L

packaging

pack of 100 g
pack of 2.5 kg
pack of 500 g

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbe id | susceptibility testing: suitable
microbiological culture: suitable

final pH

7.3±0.2 (25 °C)

application(s)

clinical testing
food and beverages
microbiology

storage temp.

10-30°C

suitability

bacteria
nonselective for Neisseria spp.
nonselective for bacteria (General Media)

Quality Level

200

相关类别

Application

Mueller Hinton琼脂是一种固体培养基，最初设计用于分离病原性奈瑟菌，现已广泛用于临床标本中分离出的需氧菌和兼性厌氧菌进行抗菌药物敏感试验（包括磺胺类药）。

General description

Mueller Hinton Agar是专为使用纸片扩散法（Kirby Bauer法）进行抗生素敏感性测试而设计。CLSI基于以下原因推荐其作为抗生素敏感性检测的理想培养基：

  • 它显示出良好的批间重现性
  • 在一些抑制剂（如三甲嘧啶、磺胺类和四环素类）中的含量较低
  • 支持需复杂和不需复杂营养的细菌病原菌的生长。
  • 培养基中的淀粉吸收毒素，从而防止其干扰抗生素的测试。
  • 该培养基为松散状琼脂，因此有助于抗生素的扩散。
Mueller Hinton琼脂已用于：
  • 培养大肠杆菌ATCC™ 25922菌株以便进行纸片扩散研究
  • 夫西地酸针对金黄色葡萄球菌、蜡样芽孢杆菌、铜绿假单胞菌和大肠杆菌的纸片扩散试验
  • 载药纳米羟基磷灰石制剂针对金黄色葡萄球菌、铜绿假单胞菌和大肠杆菌的琼脂扩散试验

Other Notes

将血液添加到 Mueller-Hinton 琼脂中，可以增强头孢噻肟和其他头孢霉素对粪肠球菌的作用
我们现提供两种类型的培养基：GranuCult®优质颗粒状培养基和NutriSelect®高性价比粉末状培养基，满足用户不同层次的需求。
商标后的品级称号basic（基础）、plus（加强）或prime（特级）代表产品的不同质控水平，从基础的质控到标准质控加强版到全面合规的特级质控水平。

Preparation Note

将38 g本品悬浮于1升蒸馏水中，煮沸，完全溶解培养基，在121°C高压灭菌15分钟。

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

法规信息

动植物来源培养基
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
BCCN3873
BCCM7657
BCCL6979
BCCL0692
BCCK3298

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Multi-drug delivery system based on lipid membrane mimetic coated nano-hydroxyapatite formulations
Placente D, et al.
International Journal of Pharmaceutics, 548(1), 559-570 (2018)
Mehmet Evren Okur et al.
Saudi pharmaceutical journal : SPJ : the official publication of the Saudi Pharmaceutical Society, 28(3), 338-348 (2020-03-21)
The objective of the present research was to examine the possible usage of microemulsion based gel for fusidic acid (FA) dermal application as burn wound treatment. During the preparation of microemulsion, ethyl oleate as oil phase, tween 80 as a
G M Eliopoulos et al.
Diagnostic microbiology and infectious disease, 12(2), 149-156 (1989-03-01)
The activities of cefotaxime and other aminothiazoyl oxime cephalosporins against Enterococcus faecalis were enhanced by addition of 5% sheep blood to Mueller-Hinton agar. This effect was not seen with aztreonam (aminothiazoyl oxime monobactam), cefotiam (aminothiazoyl, nonoxime), or other cephalosporins, and
Solid-state structure and antimicrobial and cytotoxicity studies of a cucurbit [6] uril-like Cu6L4 constructed from 3, 5-bis [(1H-tetrazol-5-yl) methyl]-4H-1, 2, 4-triazol-4-amine
Vasylevskyi S, et al.
Acta Crystallographica Section C, Structural Chemistry, 74(11) (2018)
R D Jenkins et al.
Journal of clinical microbiology, 22(3), 369-374 (1985-09-01)
Disk diffusion susceptibility tests for enterococci are frequently modified by adding 5% sheep blood (SB) to Mueller-Hinton agar; the performance standards from the National Committee for Clinical Laboratory Standards sanction this addition. Susceptibility testing of aminoglycoside antibiotics is not recommended
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