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无菌性
sterile; 0.45 μm filtered
质量水平
表单
dry powder
保质期
limited shelf life, expiry date on the label
技术
microbiological culture: suitable
溶解性
sterile water: 100 mg/mL
应用
food and beverages
microbiology
运输
ambient
储存温度
2-8°C
适用性
selective for bacteria: Yersinia enterocolitica
一般描述
Y. enterocolitica Millichrome™ plus Supplement is a selective media supplement for the detection of Yersinia enterocolitica. The distinguishing feature of these media is the inclusion of a chromogenic substrate mix that produces visible color changes upon enzymatic activity of Yersinia enterocolitica.Millichrome™ chromogenic media is a specialized type of culture medium used to detect and isolate specific groups of bacteria based on their ability to produce enzymes with chromogenic substrates. The package contains 1 vials sufficient for 1 liter of final media. The prepared supplement is added to 1 liter of melted Y. enterocolitica Millichrome™ plus Agar base cooled at 45-50 °C +/- 2 °C.
应用
Y. enterocolitica Millichrome™ plus Supplement is a selective chromogenic media supplement intended for use in the qualitative detection, differentiation, and presumptive identification of Yersinia enterocolitica from various samples.
组分
Composition per vial:
(1 vial is sufficient for 5 L of final media)
Selective mix 0.5g
(1 vial is sufficient for 5 L of final media)
Selective mix 0.5g
制备说明
- Prepare a stock solution of the supplement: Add 100 mg to 1 mL of purified water.
- Swirl well until complete dissolution. Filter sterilize at 0.45 μm.
- Warning 2: This supplement stock solution should be used immediately after Preparation, or can be stored at -20 °C and used within 15 days.
- Add 1 mL of the prepared supplement solution to 1 L of the prepared Y. enterocolitica Millichrome™ plus Agar base cooled at 45-50 °C +/- 2 °C.
- Swirl gently to homogenize.
- Pour into sterile Petri dishes.
分析说明
The Pathogenic Y. enterocolitica is detected by a characteristic mauve color after only 36-48 h of incubation, while the non-pathogenic Y. enterocolitica and background flora (Citrobacter, Enterobacter, Aeromonas, etc.) form metallic blue colonies or limited growth in metallic blue color after incubation at the same temperature
法律信息
MilliChrome is a trademark of Merck KGaA, Darmstadt, Germany
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 3
法规信息
新产品
商品
Selective media enable faster results and visual confirmation for the detection, identification, and enumeration of microorganisms
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