Agency
according to ISO 10273:2017
质量水平
无菌性
non-sterile
产品线
BioChemika
形式
powder
保质期
limited shelf life, expiry date on the label
组成
agar, 15.0 g/L
esculin, 1 g/L
ferric citrate, 0.5 g/L
meat extract, 3 g/L
meat peptone, 5 g/L
ox-bile, 40 g/L
制造商/商品名称
NutriSelect® Plus
技术
microbe id | specific enzyme detection: suitable
最终pH
6.6±0.2 (25 °C)
应用
environmental
food and beverages
water monitoring
microbiology
适用性
Streptococcus spp.
selective and differential for Enterococcus spp.
应用
Bile esculin agar (BEA) is a differential and selective medium and is mainly used to discern group D Streptococci and Enterococci based on the organism′s potential to hydrolyze esculin. BEA comprises of oxygall (bile salts, first selective ingredients) and azide (second selective ingredients), former inhibits the growth of gram positive organisms and latter inhibits the growth of gram negative organisms. BEA also comprises of nutrients ferric citrate and esculin, where esculin is a differential ingredient and is also a fluorescent compound, upon hydrolyzation the fluorescence is lost. BEA is used for bile esculin test which is based on the hydrolysis of esculin into esculetin (6, 7-dihydroxy-coumarin) and glucose by a micro-organism that produce an enzyme esculinase.
For the preliminary identification of enterococci (serology group D), as defined by Swan (1954) and Facklam and Moody (1970).
制备说明
Dissolve 64.5 g in 1 litre distilled water. Bring to a boil while stirring continually. This medium should NOT be autoclaved. Cool to 45-50°C. Mix gently and dispense into sterile Petri dishes.
附注
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
法律信息
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物来源培养基
R R Facklam et al.
Applied microbiology, 20(2), 245-250 (1970-08-01)
Six tests commonly used for the presumptive identification of group D streptococci were evaluated. Strains tested included 282 group D streptococci and 366 non-group D. Ratios of percentages of group D to non-group D strains which gave positive reactions for
Use of bile-esculin agar for rapid differentiation of Enterobacteriaceae.
Lindell, SHIRLEY S., and P. A. T. R. I. C. I. A. Quinn
Journal of Clinical Microbiology, 1 (5), 440-443 (1975)
Bile Esculin Test- Principle, Procedure, Result Interpretation and Limitation. Microbiology notes. Pratiksha Pokhrel September 1, 2015 Bile Esculin Test- Principle, Procedure, Result Interpretation and Limitation2015-10-16T17:40:41+00:00 Bacteriology, Biochemical Test, Diagnosis, Laboratory.
Science (2015)
The use of a bile-aesculin medium and of Maxted's technique of Lancefield grouping in the identification of enterococci (group D streptococci).
A SWAN
Journal of clinical pathology, 7(2), 160-163 (1954-05-01)
商品
Microbiological assessment of bacteria found in spices, a potent source for food spoilage and pathogens.
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