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安全信息

21819

Sigma-Aldrich

按照 Kinyoun 的石碳酸复红溶液

for microscopy

别名:

Kinyoun 石碳酸复红

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About This Item

MDL编号:
MFCD00143924
UNSPSC代码:
12171500
PubChem化学物质编号:
329752209
NACRES:
MA.02

等级

for microscopy

质量水平

100

表单

liquid

组成

fuchsin basic (certified stain; C.I. 42500), 3 % (w/w)

技术

microbe id | staining: suitable

杂质

phenol

颜色

red to very dark red

密度

0.99 g/mL at 20 °C

ε (消光系数)

77 at 546 nm in 50% ethanol

应用

diagnostic assay manufacturing
hematology
histology

储存温度

room temp

SMILES字符串

NC1=CC=C(C(C2=CC=C(N)C=C2)=C(C=C3)C=CC3=[NH2+])C=C1.[Cl-]

InChI

1S/C21H21N3/c1-13-11-16(5-9-19(13)23)21(15-3-7-18(22)8-4-15)17-6-10-20(24)14(2)12-17/h3-12,22H,23-24H2,1-2H3

InChI key

IMOKRVRFBCWWGH-UHFFFAOYSA-N

相关类别

一般描述

Carbol-Fuchsin is an acid-fast stain that consists of basic fuchsin and phenol. It is a dry staining dye for staining acid-fast bacteria such as Mycobacterium tuberculosis. Carbol-Fuchsin solution according to Kinyoun is a modified stain that does not require heat to infuse the carbol-fuchsin into the acid-fast cells during the staining process, and this process is also known as “cold staining”. It requires a higher concentration of the primary stain.

应用

  • Kinyoun’s carbol-fuchsin is intended for use to stain paraffin sections of a formaldehyde-fixed specimen containing tubercle bacilli, and for the demonstration of tuberculosis bacteria (Mycobacterium tuberculosis).
  • It is also applicable for other organisms that retain the dye after immersion in acidified 70% alcohol.
  • It has been used to develop a staining technique for detecting microsporidial spores in fecal specimens.

原理

Acid-fast bacteria possess a waxy, lipid-rich cell wall that absorbs dyes very slowly. To enhance the absorption of carbol-fuchsin dye and the subsequent formation of the mycolate-fuchsin complex, the dye solution applied to the specimen is normally heated to the evaporation point. In Kinyoun’s modification of this method, the heating of the solution is avoided. Consequently, hazardous phenolic vapors are not released.

其他说明

用于显示肺结核菌的染色剂

警示用语：

Danger

危险分类

Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Skin Corr. 1B

储存分类代码

3 - Flammable liquids

WGK

WGK 3

闪点(°F)

109.9 °F - closed cup

闪点(°C)

43.3 °C - closed cup

法规信息

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分析证书(COA)

Lot/Batch Number
BCCM3860
BCCL4681
BCCL3237
BCCL7129
BCCK9547

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L. Hallmann et al.
Klinische Mikrobiologie null
Ritu Singhal et al.
International journal of mycobacteriology, 4(1), 1-6 (2015-12-15)
Tuberculosis continues to cast a huge impact on humanity with its high incidence and mortality, especially in developing countries. For tuberculosis case detection, microscopy continues to be indispensible, given its low cost, rapidity, simplicity of procedure and high specificity. Modifications
Fatmah M Salleh et al.
Diagnostic microbiology and infectious disease, 69(1), 82-85 (2010-12-15)
This study was conducted to evaluate the modification of the usual Gram-chromotrope staining technique developed in-house known as Gram-chromotrope Kinyoun (GCK) in comparison with the Weber Modified Trichrome (WMT) staining technique; as the reference technique. Two hundred and ninety fecal
Evaluation of gram-chromotrope kinyoun staining technique: its effectiveness in detecting microsporidial spores in fecal specimens
Salleh, et al.
Diagnostic Microbiology and Infectious Disease, 69, 82-85 (2011)

商品

Detection & Molecular Identification of Mycobacteria

Identifying Mycobacterium species crucial due to varying clinical significance; some pathogenic, others not.

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