等级
for microscopy
质量水平
形式
liquid
组成
fuchsin basic (certified stain; C.I. 42500), 3 % (w/w)
杂质
phenol
颜色
red to very dark red
密度
0.99 g/mL at 20 °C
ε (消光系数)
77 at 546 nm in 50% ethanol
应用
diagnostic assay manufacturing
hematology
histology
储存温度
room temp
SMILES字符串
NC1=CC=C(C(C2=CC=C(N)C=C2)=C(C=C3)C=CC3=[NH2+])C=C1.[Cl-]
InChI
1S/C21H21N3/c1-13-11-16(5-9-19(13)23)21(15-3-7-18(22)8-4-15)17-6-10-20(24)14(2)12-17/h3-12,22H,23-24H2,1-2H3
InChI key
IMOKRVRFBCWWGH-UHFFFAOYSA-N
一般描述
Carbol-Fuchsin is an acid-fast stain that consists of basic fuchsin and phenol. It is a dry staining dye for staining acid-fast bacteria such as Mycobacterium tuberculosis. Carbol-Fuchsin solution according to Kinyoun is a modified stain that does not require heat to infuse the carbol-fuchsin into the acid-fast cells during the staining process, and this process is also known as “cold staining”. It requires a higher concentration of the primary stain.
应用
- Kinyoun’s carbol-fuchsin is intended for use to stain paraffin sections of a formaldehyde-fixed specimen containing tubercle bacilli, and for the demonstration of tuberculosis bacteria (Mycobacterium tuberculosis).
- It is also applicable for other organisms that retain the dye after immersion in acidified 70% alcohol.
- It has been used to develop a staining technique for detecting microsporidial spores in fecal specimens.
原理
Acid-fast bacteria possess a waxy, lipid-rich cell wall that absorbs dyes very slowly. To enhance the absorption of carbol-fuchsin dye and the subsequent formation of the mycolate-fuchsin complex, the dye solution applied to the specimen is normally heated to the evaporation point. In Kinyoun’s modification of this method, the heating of the solution is avoided. Consequently, hazardous phenolic vapors are not released.
其他说明
用于显示肺结核菌的染色剂
警示用语：
Danger
危险分类
Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Skin Corr. 1B
WGK
WGK 3
闪点(°F)
closed cup
闪点(°C)
closed cup
法规信息
新产品
Fatmah M Salleh et al.
Diagnostic microbiology and infectious disease, 69(1), 82-85 (2010-12-15)
This study was conducted to evaluate the modification of the usual Gram-chromotrope staining technique developed in-house known as Gram-chromotrope Kinyoun (GCK) in comparison with the Weber Modified Trichrome (WMT) staining technique; as the reference technique. Two hundred and ninety fecal
L. Hallmann et al.
Klinische Mikrobiologie null
Evaluation of gram-chromotrope kinyoun staining technique: its effectiveness in detecting microsporidial spores in fecal specimens
Salleh, et al.
Diagnostic Microbiology and Infectious Disease, 69, 82-85 (2011)
Ritu Singhal et al.
International journal of mycobacteriology, 4(1), 1-6 (2015-12-15)
Tuberculosis continues to cast a huge impact on humanity with its high incidence and mortality, especially in developing countries. For tuberculosis case detection, microscopy continues to be indispensible, given its low cost, rapidity, simplicity of procedure and high specificity. Modifications
商品
Identifying Mycobacterium species crucial due to varying clinical significance; some pathogenic, others not.
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