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Merck
CN

HKG6PDH-RO

Roche

己糖激酶/葡萄糖-6-磷酸脱氢酶(HK / G6P-DH)

from yeast/Leuconostoc, overproducer

别名:

G6P-DH, 己糖激酶, 葡萄糖-6-磷酸脱氢酶HK

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About This Item

UNSPSC代码:
12352204

生物来源

yeast

质量水平

形式

suspension

分子量

dimer 110,000 Da

包装

pkg of 10 mL (10737275001 [30 mg])
pkg of 5 mL (10127825001 [15 mg])

制造商/商品名称

Roche

浓度

3.21 mg/mL

技术

activity assay: suitable

颜色

white

pH值(酸碱度)

5.5-6.5

溶解性

water: miscible

适用性

suitable for UV spectrophotometry and general use

应用

life science and biopharma

储存温度

2-8°C

一般描述

己糖激酶负责催化糖酵解的第一步。它导致葡萄糖和 ATP 形成葡萄糖-6-磷酸和 ADP。 葡萄糖-6-磷酸脱氢酶是磷酸戊糖途径中的关键酶。负责葡萄糖-6-磷酸和 NADP +(烟酰胺腺嘌呤二核苷酸磷酸)转化为 6-磷酸葡萄糖酸内酯和 NADPH。

应用

己糖激酶/6-磷酸葡萄糖脱氢酶已用于测定血浆葡萄糖水平。

组分

EC 2.7.1.1 & 1.1.1.49

单位定义

在+ 25°C和pH 7.6条件下,1个单位(U)HK将在1分钟内磷酸化1μmol的D-葡萄糖。
在+ 25℃和pH 7.6条件下,1单位(U)G6P-DH将在1分钟内氧化1μmol葡萄糖-6-磷酸。
在偶联试验中,每μmolD-葡萄糖磷酸化产生1μmolNADH。

体积活性:以葡萄糖和ATP为底物,+ 25℃时产生340u己糖激酶/ml。170 U葡萄糖-6-磷酸脱氢酶/ ml(+ 25°C,葡萄糖-6-磷酸为底物)

外形

用 3.2 M 硫酸铵溶液(pH 值约为 6)混悬。己糖激酶与 G6P-DH 混合制备。HK:G6P-DH的蛋白质含量约为2:1。

其他说明

仅用于生命科学研究。不用于诊断程序。

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

does not flash

闪点(°C)

does not flash

法规信息

常规特殊物品

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