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Merck
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COLLDISP-RO

Roche

胶原酶/中性蛋白酶®

lyophilized, non-sterile, optimum pH 7.0-8.0

别名:

胶原蛋白酶

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UNSPSC Code:
12352204
NACRES:
NA.54

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产品名称

胶原酶/中性蛋白酶®, lyophilized, non-sterile, optimum pH 7.0-8.0

biological source

enzyme from Bacillus polymyxa ( Dispase )
enzyme from bacterial ( Collagenase from Vibrio alginolyticus)

sterility

non-sterile

form

lyophilized

collagenase activity

>0.1 U/mg lyophilizate, 25 °C (4-phenyl-azobenzyl-oxycarbonyl-Pro-Leu-Gly-Pro-D-Arg as substrate)

specific activity

>0.8 U/mg lyophilizate, pH 7.5, 37 °C (casein as substrate, Dispase)

packaging

pkg of 100 mg (10269638001)
pkg of 500 mg (11097113001)

manufacturer/tradename

Roche

concentration

1 mg/mL (Collagenase: 0.1U/mL, Dispase: 0.8U/mL)

technique(s)

tissue processing: suitable

color

light yellow to brown

optimum pH

7.0-8.0

solubility

water: soluble

UniProt accession no.

P43154

application(s)

life science and biopharma
sample preparation

foreign activity

proteolytic activities <0.1 U/mg (hemoglobin as the substrate)

storage temp.

2-8°C

Quality Level

100

相关类别

Application

胶原酶/中性蛋白酶® 用于从多种不同的组织和器官中制备细胞。

Biochem/physiol Actions

胶原酶降解天然胶原蛋白。中性蛋白酶® 是一种非特异性的金属蛋白酶。

General description

分散酶是一种中性、快速反应、温和的蛋白酶，能将表皮从真皮中完整分离。它还能将培养的上皮细胞从基质中完整分离出来。其可分裂基底膜区，使上皮细胞保持完整。胶原酶是一种分解三螺旋蛋白胶原蛋白的蛋白酶。组织胶原酶分三种类型，它们属于基质金属蛋白酶(MMP)家族。从溶组织性梭状芽孢杆菌中提取的胶原酶具有很强的活性，因为它可在低至4-10℃的温度下，从两端消化胶原蛋白。

Other Notes

EC 3.4.24.4 & 3.4.24.3
一个单位是指，在1分钟内在反应条件下释放出1 μmol酪氨酸对应的Folin-阳性氨基酸和多肽所需的酶活性。
仅用于生命科学研究。不可用于诊断。
胶原酶来源于 溶藻弧菌, 中性蛋白酶® 来源于 多粘芽孢杆菌

Preparation Note

激活剂：Ca2+

工作浓度：约为1mg/mL（胶原酶：0.1U/mL，中性蛋白酶：0.8U/mL

酶活性：
胶原酶：0.1 Wünsch U/mg冻干粉（+ 25°C，4-苯基-偶氮苄基-氧基羰基-Pro-Leu-Gly-Pro-D-Arg作为底物）
中性蛋白酶®：0.8 Wünsch U/mg冻干粉（+ 37°C，酪蛋白为底物，pH 7.5）
污染蛋白水解活性：<0.1U/mg 冻干粉 (血红蛋白作为底物)

工作溶液：工作溶液制备 将酶溶于水中，使其终浓度为100mg/mL。用PBS(不含Ca2+/Mg2+)稀释至终浓度为1mg/mL，并采用无菌0.2μm 过滤膜过滤。
吴军溶液的酶浓度：胶原酶，0.1U/mL PBS; 中性蛋白酶®, 0.8U/mL PBS。

抑制剂：
胶原酶抑制剂：EDTA, EGTA
血清不能抑制胶原酶/中性蛋白酶。

保存条件（工作溶液）：-15 至 -25°C
复溶的储备溶液在-15至-25°C下稳定。
注：适当分装保存并避免反复冻融！
复溶于水中（100mg/mL）后续稀释采用PBS(磷酸缓冲盐)(不含Ca2+/Mg2+)。
干燥储存

Legal Information

Dispase is a registered trademark of Godo Shusei Co., Ltd.

pictograms

Exclamation markHealth hazard
GHS07,GHS08

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

存储类别

11 - Combustible Solids

wgk

WGK 1

flash_point_f

does not flash

flash_point_c

does not flash

法规信息

常规特殊物品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
73680321
68654621
63837023
59985623
57028122

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Product Information Sheet-colldisp-ro

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

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