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用途
sufficient for ≤1,000 tests
质量水平
制造商/商品名称
Roche
技术
ELISA: suitable
检测方法
chemiluminescent
运输
wet ice
储存温度
2-8°C
一般描述
通过测量DNA合成期间的BrdU掺入而定量细胞增殖的化学发光免疫分析方法： [3H]-胸苷掺入测定的非放射性替代方法。
特异性
抗体结合物与胸苷类似物5-溴-2′-脱氧尿苷（BrdU）以及掺入DNA的BrdU反应。为结合掺入DNA的BrdU，必须使BrdU标记的DNA变性。抗体不与胸苷、尿苷或DNA等任何内源性细胞组分发生交叉反应。
应用
细胞增殖ELISA，BrdU（化学发光法）属于第二代改良型DNA合成检测试剂盒。它是一个精确、快速且简单的非放射性替代方法，通过测量复制（循环）细胞中DNA合成期间的BrdU掺入而定量细胞增殖。因此，细胞增殖ELISA可用于许多不同的 体外 细胞系统。例如：
- 检测和定量由生长因子和细胞因子诱导的细胞增殖
- 在环境和生物医学研究以及食品、化妆品和制药行业中，用于检测各种化合物细胞增殖的抑制或刺激作用
- 测量由有丝分裂原或抗原刺激的淋巴细胞的免疫反应性
- 在医学研究中分析肿瘤细胞对不同细胞抑制剂的化疗敏感性
细胞增殖ELISA，BrdU（化学发光法）已用于：
- ReN和原代星形胶质细胞的溴脱氧尿苷(BrdU)增殖检测
- 通过BrdU掺入实验分析伊马替尼对神经母细胞瘤细胞增殖的影响
- 通过BrdU掺入实验研究帕博西尼对胰腺病患原代细胞系中细胞周期的抑制作用
包装
1个试剂盒包含7种组分。
组分
- BrdU 标记试剂 1,000倍 浓度
- FixDenat
- 抗-BrdU-POD 4.抗体稀释溶液
- PBS 洗涤缓冲溶液, 10倍浓度
- 底物组分 A
- 底物组分 B
其他说明
仅用于生命科学研究。不可用于诊断。
仅试剂盒组分
产品编号
说明
- BrdU Labeling Reagent
- FixDenat ready-to-use
- Anti-BrdU-peroxidase antibody
- Antibody Dilution Solution ready-to-use
- ready-to-use 10x concentrated
- Substrate Component A
- Substrate Component B
警示用语：
Danger
危险分类
Eye Irrit. 2 - Flam. Liq. 2 - Muta. 1B - Skin Sens. 1
WGK
WGK 1
法规信息
含少量动物源组分生物产品
常规特殊物品
危险化学品
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基于细胞的细胞增殖检测（BrdU、MTT、WST1）、细胞活力和细胞毒性实验，用于癌症、神经科学和干细胞研究应用。
Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
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