推荐产品
用途
sufficient for ≤100 tests
制造商/商品名称
Roche
储存温度
−20°C
1 of 4
此商品 | B0631 | B9285 | B5002 |
|---|---|---|---|
| manufacturer/tradename Roche | manufacturer/tradename - | manufacturer/tradename - | manufacturer/tradename - |
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| usage sufficient for ≤100 tests | usage - | usage - | usage - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
一般描述
免疫组织化学法检测 5-溴-2′-脱氧尿嘧啶核苷 (BrdU)掺入细胞 DNA。
可通过监测放射性同位素 [ 3 H]-胸苷掺入细胞 DNA，然后进行放射自显影来研究细胞增殖。
或者，5-溴-2′-可用尿苷 (BrdU) 替代胸苷。使用针对 BrdU 的单克隆抗体和酶或荧光染料结合的第二抗体，很容易检测到 BrdU 掺入 DNA 的细胞。
或者，5-溴-2′-可用尿苷 (BrdU) 替代胸苷。使用针对 BrdU 的单克隆抗体和酶或荧光染料结合的第二抗体，很容易检测到 BrdU 掺入 DNA 的细胞。
特异性
抗 BrdU 单克隆抗体与 5-溴-2′-脱氧尿苷特异性结合，显示与 5-碘-2′ -脱氧尿苷有交叉反应 (10%)。抗 BrdU 抗体与 5-氟-2′ -脱氧尿苷或任何内源性细胞成分，如胸苷或尿苷无交叉反应。
应用
特点和优势
- 安全： 未使用放射性同位素。
- 易于执行： 遵循标准免疫组化方案。
- 灵敏： 用核酸酶使 DNA 变性可高灵敏度检测 BrdU。
- 灵活： 允许双标记方案。
包装
1个试剂盒包含7种组分。
原理
预先用 BrdU 标记的样品用乙醇固定，然后用 BrdU 单克隆抗体孵育，该抗体含有优化的核酸酶混合物。这些核酸酶产生单链 DNA 片段，允许抗体与 BrdU 结合。接着，加入碱性磷酸酶 (AP) 标记的小鼠免疫球蛋白抗体，然后与抗 BrdU 抗体结合。然后将样品与 AP 底物和代谢形成有色反应产物的 NBT/BCIP 一起孵育。使用相差显微镜评价样品。
制备说明
标记试剂的工作浓度： 标记试剂的工作浓度相当于 原位 细胞增殖试剂盒的工作浓度。
样品材料：
贴壁细胞、悬浮细胞、器官或外植体培养。体内 标记后的冰冻或石蜡包埋组织切片。
样品材料：
贴壁细胞、悬浮细胞、器官或外植体培养。体内 标记后的冰冻或石蜡包埋组织切片。
其他说明
仅用于生命科学研究。不可用于诊断。
仅试剂盒组分
产品编号
说明
- BrdU Labeling Reagent 1,000x concentrated
- Washing Buffer concentrate 10x concentrated
- Incubation Buffer
- Anti-BrdU antibody, contains nucleases for DNA denaturation
- Anti-mouse Ig-alkaline Phosphatase antibody
- NBT
- BCIP
警示用语：
Danger
危险分类
Acute Tox. 4 - Acute Tox. 4 Inhalation - Eye Irrit. 2 - Flam. Liq. 3 Dermal - Muta. 1B - Repr. 1B - Skin Sens. 1
储存分类代码
3 - Flammable liquids
WGK
WGK 2
闪点(°F)
136.4 °F
闪点(°C)
58 °C
法规信息
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商品
基于细胞的细胞增殖检测（BrdU、MTT、WST1）、细胞活力和细胞毒性实验，用于癌症、神经科学和干细胞研究应用。
Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
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