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About This Item
经验公式（希尔记法）:
C14H28O6
CAS号:
分子量:
292.37
Beilstein:
84118
MDL编号:
UNSPSC代码:
12352200
PubChem化学物质编号:
推荐产品
方案
99% (GC)
分子量
micellar avg mol wt 25,000
292.4
包装
pkg of 10 g
制造商/商品名称
Roche
聚集数
84
技术
dialysis: suitable
CMC
20-25 mM (20-25°C)
转变温度
cloud point >100 °C
运输
ambient
SMILES字符串
CCCCCCCCO[C@@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O
InChI
1S/C14H28O6/c1-2-3-4-5-6-7-8-19-14-13(18)12(17)11(16)10(9-15)20-14/h10-18H,2-9H2,1H3/t10-,11-,12+,13-,14-/m1/s1
InChI key
HEGSGKPQLMEBJL-RKQHYHRCSA-N
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一般描述
1-O-n-Octyl-β-D-glucopyranoside, octylglucoside, is a detergent with an octyl chain as hydrophobic residue and glucose as hydrophilic part. It is a non-ionic, dialyzable, mild and non-denaturing detergent which is used for the solubilization, isolation and reconstitution of membrane proteins.
应用
N-Octylglucoside has been shown to increase the resolution of proteins in 2D gels. It is a mild and non-denaturing detergent for the solubilization and reconstitution of membrane proteins. N-Octylglucoside is easily removed by dialysis. N-Octylglucoside has been used in mass spectrometric immunoassay to homogenize MALDI (matrix assisted laser desorption/ionization) matrix draw and elution.
用于溶解和分离膜蛋白的非离子型可透析洗涤剂。已证明可提高 2D 凝胶中蛋白质的分离度。
质量
Purity: 99% (from C), homogeneous (GC)
Ease of removal*: ++
CMC**: 14.5 mM at +25°C
Formula: C14H28O6
Ease of removal*: ++
CMC**: 14.5 mM at +25°C
Formula: C14H28O6
制备说明
Working concentration: 46 mM
Working solution: Solubility: > 50% (w/v) in double-dist. water, Tris-HCl 0.05 mol/l; pH7.4 or K-phosphate buffer 0.1 mol/l, pH 7.0 at 25 °C.
Storage conditions (working solution): Stability in Solution
A stock solution is stable at 2 to 8 °C for approx. 3 days. We would expect a long term stability of 6-12 months if stored frozen in portions at -15 to -25 °C, provided bacterial contamination is avoided.
Working solution: Solubility: > 50% (w/v) in double-dist. water, Tris-HCl 0.05 mol/l; pH7.4 or K-phosphate buffer 0.1 mol/l, pH 7.0 at 25 °C.
Storage conditions (working solution): Stability in Solution
A stock solution is stable at 2 to 8 °C for approx. 3 days. We would expect a long term stability of 6-12 months if stored frozen in portions at -15 to -25 °C, provided bacterial contamination is avoided.
储存及稳定性
Store at 15 to 25 °C. (Store dry!)
其他说明
For life science research only. Not for use in diagnostic procedures.
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
监管及禁止进口产品
W A Petri et al.
The Journal of biological chemistry, 254(11), 4313-4316 (1979-06-10)
The glycoprotein of vesicular stomatitis (VS) virus was selectively liberated from the virion membrane by the dialyzable nonionic detergent, beta-D-octylglucoside. The isolated viral glycoprotein could be rendered virtually free of phospholipid and detergent, under which conditions it formed tail-to-tail glycoprotein
Howard R Mellor et al.
The Biochemical journal, 374(Pt 2), 307-314 (2003-05-29)
The N-alkyl moiety of N-alkylated imino sugars is crucial for therapeutic activities of these compounds as inhibitors of glycosphingolipid (GSL) biosynthesis and as antivirals. The improved potency afforded by a long N-alkyl moiety is coincident with increased compound-induced cytotoxicity. Therefore
Olgica Trenchevska et al.
Journal of proteome research, 9(11), 5969-5973 (2010-09-09)
Protein biomarkers are essential in assessing pathogenic processes. The impetus for finding new biomarkers has been accelerated by the arrival of the "omics" technologies. However, equally important is the rediscovery of existing biomarkers with these new approaches as novel variants
Olgica Trenchevska et al.
Proteome science, 9(1), 19-19 (2011-04-12)
Post-translational modifications and genetic variations give rise to protein variants that significantly increase the complexity of the human proteome. Modified proteins also play an important role in biological processes. While sandwich immunoassays are routinely used to determine protein concentrations, they
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