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Merck
CN

WBAVDCH01

Immobilon® 封闭液 - CH(化学发光封闭液)

Chemiluminescent detection kit used for Western blotting, dot/slot blotting

别名:

Western blot blocking buffer, blocking buffer

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UNSPSC Code:
41116010
NACRES:
NB.22
eCl@ss:
42029053
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产品名称

Immobilon® 封闭液 - CH(化学发光封闭液), Chemiluminescent detection kit used for Western blotting, dot/slot blotting

conjugate

peroxidase conjugate

packaging

pkg of 500 mL

manufacturer/tradename

Immobilon®

technique(s)

western blot: suitable

membrane area

1000 cm2 , membrane coverage

detection method

chemiluminescent

shipped in

ambient

storage temp.

15-25°C

Quality Level

Analysis Note

为了获得最佳结果,使用Immobilon-P膜进行化学发光(鲁米诺)或目视检测(例如TMB),并使用Immobilon-FL膜进行荧光检测。

Application

用于免疫印迹检测。

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

General description

Immobilon® Block降噪试剂是专门为蛋白质印迹应用而设计的。该系列无蛋白即用型缓冲液经过优化,可在使用化学发光,荧光或磷酸酪氨酸检测时降低背景水平。Bløk缓冲液非常适合与Immobilon-P蛋白质印迹膜和SNAP i.d.系统一起使用,并且还与标准的蛋白质印迹技术兼容。

Packaging

足够的试剂用于19次迷你印迹(8x7 cm)

Preparation Note

在20–25°C条件下可稳定保存2年

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Aquatic Chronic 3 - Skin Sens. 1

存储类别

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Tabetha Sundin et al.
Biochimie, 94(12), 2639-2648 (2012-08-21)
Isoprenoids are recognized for their ability to suppress carcinogenic processes in vivo and in vitro. We previously established that the isoprenoid, perillyl alcohol, acted mechanistically on translation of specific proteins through modulation of mechanistic target of rapamycin (mTOR) signaling. Telomerase-the

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There’s so much room for experimental variability in traditional immunodetection workflows. For your peace of mind – and ours – we designed the SNAP i.d.® 2.0 system to streamline your Western blot and immunohistochemistry experiments. The concept is simple: a vacuum-driven flow of blocking, antibody, and washing solutions reduces slide and membrane handling. That means a lot less shaking, dipping, pouring and waiting. And now you can process multiple blots and slides in parallel, so it’s easy to apply consistent conditions across experiments.

Antibody reuse for Western blotting is a common practice for many researchers. While many antibodies lose potency with time or degrade even faster due to improper storage conditions, it is important to recognize the potential value of recovering the primary antibody for possible reuse in some experiments. The SNAP i.d.® 2.0 system is not only able to reduce the immunodetection processing time, but its flexibility lets you combine conditions used in the standard immunodetection protocol and also allows the collection of antibody for future reuse. Here, we compare the antibody recovery and reuse in the standard immunodetection protocol with the antibody recovery and reuse in SNAP i.d.® system using the extended protocol and the original SNAP i.d.® protocol.

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