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SCT010M

Sigma-Aldrich

LuminiCell Tracker 540- Cell Labeling Kit

Biocompatible organic fluorescent nanoparticles (AIEDots) that can be used to label cells for long term cell tracking and cell tracing in vitro and in vivo experiments.

别名:

AIEDots Cell Tracker Dye, AIE Live Cell Fluorescent Dye, Fluorescent Cell Tracking Dye, Live Cell Fluorescent Nanoparticles

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25 EA
CN¥577.48

CN¥577.48

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25 EA
CN¥577.48

About This Item

UNSPSC代码:
12352207
eCl@ss:
32161000
NACRES:
NA.47

CN¥577.48

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质量水平

100

技术

cell culture | mammalian: suitable

检测方法

fluorometric

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HCVD1MAG67K

MILLIPLEX® Human Cardiovascular Disease (CVD) Magnetic Bead Panel 1 - Cardiovascular Disease Multiplex Assay

MILLIPLEX® Human Cardiovascular Disease (CVD) Magnetic Bead Panel 4 - Cardiovascular Disease Multiplex Assay The analytes available for this multiplex kit are: sE-Selectin, Follistatin, Pecam-1, Pentraxin-3, Tissue Factor (TF), Thrombomodulin, Troponin T (TnT).

HCVD4MAG67K

MILLIPLEX® Human Cardiovascular Disease (CVD) Magnetic Bead Panel 4 - Cardiovascular Disease Multiplex Assay

MILLIPLEX® Human Cardiovascular Disease (Acute Phase) Magnetic Bead Panel 3 - Cardiovascular Disease Multiplex Assay The analytes available for this multiplex kit are: Adipsin, AGP, α2-Macroglobulin, CRP, Fetuin A, Fibrinogen, L-Selectin, Serum Amyloid P, Haptoglobin, & Platelet Factor-4.

HCVD3MAG67K

MILLIPLEX® Human Cardiovascular Disease (Acute Phase) Magnetic Bead Panel 3 - Cardiovascular Disease Multiplex Assay

MILLIPLEX® Human Cardiovascular Disease (CVD) Magnetic Bead Panel 2 - Cardiovascular Disease Multiplex Assay The analytes available for this multiplex kit are: ADAMTS13, D-Dimer, GDF-15, Myoglobin, sICAM-1, MPO, P-selectin, Lipocalin-2/NGAL, sVCAM-1, SAA.

HCVD2MAG67K

MILLIPLEX® Human Cardiovascular Disease (CVD) Magnetic Bead Panel 2 - Cardiovascular Disease Multiplex Assay

assay range

accuracy: 100%
(CK-MB), accuracy: 102%
(Oncostatin (OSM)), accuracy: 96%
(FABP3), accuracy: 97%
(NT proBNP), standard curve range: 2.7-2,000 pg/mL
(Placental Growth Factor (PlGF)), standard curve range: 6.9-5,000 pg/mL
(CXCL6, Oncostatin (OSM)), standard curve range: 685.9-500,000 pg/mL
(FABP4), inter-assay cv: <20%
intra-assay cv: <10%, accuracy: 103%
(CXCL6/GCP-2), standard curve range: 137.2-100,000 pg/mL
(FABP3), standard curve range: 68.6-50,000 pg/mL
(BNP, CK-MB, Troponin I (TnI)), accuracy: 102%
(LIGHT), standard curve range: 34.3-25,000 pg/mL
(NT proBNP, CXCL16, Endocan-1 (ESM-1), LIGHT), accuracy: 96%
(Placental Growth Factor (PlGF))

assay range

accuracy: 103%
(Thrombomodulin), accuracy: 104%
(Pentraxin-3 (PTX3)), accuracy: 104%
(Tissue Factor (TF)), accuracy: 106%
(Pecam-1), accuracy: 109%
(sE-Selectin), standard curve range: 0.01-5 ng/mL
(Troponin T (TnT)), standard curve range: 0.04-30 ng/mL
(Follistatin (FST)), standard curve range: 0.14-100 ng/mL
(Pentraxin-3 (PTX3) & Tissue Factor (TF)), standard curve range: 0.27-200 ng/mL
(Pecam-1), standard curve range: 0.34-250 ng/mL
(Thrombomodulin), standard curve range: 2.7-2,000 ng/mL
(sE-Selectin), inter-assay cv: <20%
intra-assay cv: <10%

assay range

accuracy: 102%
(Haptoglobin), accuracy: 109%
(AGP), accuracy: 113%
(Platelet Factor-4), accuracy: 117%
(Adipsin), standard curve range: 0.004-15 ng/mL
(L-Selectin & SAP), standard curve range: 0.012-50 ng/mL
(CRP), standard curve range: 0.037-150 ng/mL
(PF4), standard curve range: 0.061-250 ng/mL
(HPTGN), standard curve range: 0.098-400 ng/mL
(AGP & Adipsin), standard curve range: 0.244-1,000 ng/mL
(Fetuin A & vWF), standard curve range: 0.488-2,000 ng/mL
(A2M)

assay range

accuracy: 88%
(Myoglobin), accuracy: 90%
(P-selectin), accuracy: 98%
(GDF-15), standard curve range: 0.0005-2.5 ng/mL
(GDF-15), standard curve range: 0.012-50 ng/mL
(Lipocalcin-2/NGAL), standard curve range: 0.024-100 ng/mL
(Myoglobin & MPO), standard curve range: 0.085-350 ng/mL
(sICAM-1), standard curve range: 0.122-500 ng/mL
(sVCAM-1), standard curve range: 0.244-1,000 ng/mL
(D-dimer, SAA & P-selectin), standard curve range: 1.221-5,000 ng/mL
(ADAMTS 13), inter-assay cv: <20%
intra-assay cv: <15%

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

manufacturer/tradename

Milliplex®

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

technique(s)

multiplexing: suitable

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

一般描述

Absorbance Maximum: 423nm
Emisson Maximum: 540nm

Suggested Filters:
Excitation: 405/458/488nm
Emisson: 480-560nm
Long-term noninvasive cell tracking by fluorescent probes and quantum dots is of great importance to life science and biomedical engineering. Current methods used to fluorescently tag cells have been limited by short signal duration, high background auto-fluorescence or lengthy molecular cloning manipulations using GFP. LuminiCell Trackers<TMSYMBOL></TMSYMBOL> are biocompatible organic fluorescent nanoparticles based on Aggregation Induced Emission technology, termed AIEDots. Aggregation induced emission (AIE) molecules emit fluorescence in an opposite manner than other common fluorophores (Quantum Dots, GFP). Propeller-shaped AIE fluorogens are non-emissive in solutions but become highly fluorescent upon aggregate formation. Due to these differences, LuminiCell Trackers<TMSYMBOL></TMSYMBOL> have very high fluorescence intensities with minimal signal quenching allowing live cell fluorescent tagging for up to 10 days in vitro and 21 days in vivo. These properties make them optimal candidates for long interval live cell bioimaging experiments.

The Luminicell Tracker<TMSYMBOL></TMSYMBOL>-540 Cell Labeling Kit contain green fluorescent AIEDot nanoparticles with TAT sequences. These nanoparticles can be used to fluorescently tag cells for long term cell tracking and tracing experiments.

Features and Benefits

● Brighter: 10X brighter than other cell labeling technologies
● Photostable: 3X longer fluorescence without signal quenching
● Biocompatible: Non-toxic organic nanoparticles intended for biological applications
● Rapid Protocol: Easy-to-use protocol labels cells within 4 hours

应用

Biocompatible organic fluorescent nanoparticles (AIEDots) that can be used to label cells for long term cell tracking and cell tracing in vitro and in vivo experiments.

质量

Absorbance: 422+-5nm
Concentration: 180-220nM
Fluorescence: 540+/- 10nm
Quantum Yield: ≥50%
Brightness at 540nM: ≥3X10^7 M-1cm-1
Cellular Assay: HeLa Cell Fluorescence

外形

20nM in 0.5ml PBS, pH 7.4

储存及稳定性

Store at -2-8°C upon receipt. Thaw at room temperature or in a water bath. Do not Freeze.

Note: Some particulates may form as a result of nanoparticle aggregation during shipping. To get particulates back in solution, sonicate the vial containing LuminiCell Tracker three times for 1 min each before use.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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