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Merck
CN

S2EM004M99

Multiscreen® 96孔板,疏水性PVDF膜

pore size 0.45 μm, sterile

别名:

PVDF 96孔板, 疏水性96孔板

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UNSPSC Code:
41104923
NACRES:
NB.22
eCl@ss:
32039006
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产品名称

Multiscreen® 96孔板,疏水性PVDF膜, pore size 0.45 μm, sterile

description

Sterile, white 96-well filter plate with 0.45 um pore size Hydrophobic PVDF membrane for ELISPOT Assays. Comes in a pack of 10.

sterility

sterile

Quality Level

material

Durapore PVDF membrane
acrylic plate

usage

Manual

packaging

pkg of 10 plates

manufacturer/tradename

MultiScreen®

matrix

Immobilon®-P

pore size

0.45 μm pore size

compatibility

manifold only

shipped in

ambient

technique(s)

ELISPOT: suitable
cell based assay: suitable

filtration area

0.28 cm2

plate size

96 wells

working volume

50-250 μL

Application

MultiScreen®-IP 白色无菌过滤板, 0.45 μm已被用于酶联免疫斑点(ELISPOT)试验。

General description

滤器编码:IP

Other Notes

自动化兼容性:否

Physical form

色码:白色

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany
MULTISCREEN is a registered trademark of Merck KGaA, Darmstadt, Germany

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Sung-Ho Chang et al.
Cells, 9(5) (2020-05-24)
We investigated the immunogenicity of allogeneic human adipose-derived mesenchymal stem cells (ADSCs) through the production of alloreactive-CD8 T and -memory CD8 T cells, based on their human leukocyte antigen (HLA) expression. In surface antigen analysis, ADSCs do not express co-stimulatory
Sarah Côté-Bigras et al.
Clinical endocrinology, 80(2), 296-300 (2013-06-06)
While thyrotrophin receptor (TSHR) is recognized as the main autoantigen in Graves' disease (GD), the actual antigen specificity of T cells that infiltrate the thyroid and the orbit is unknown. Identifying T cell responses to TSHR peptides has been difficult
Daniela Frasca et al.
Vaccine, 34(25), 2834-2840 (2016-04-25)
The success of a vaccine in inducing a protective antibody response depends on the longevity of both long-lived plasma cells (PC) and memory B cells. We have previously shown that the in vivo antibody response to a new influenza vaccine
Kee Thai Yeo et al.
Journal of immunology (Baltimore, Md. : 1950), 202(5), 1465-1478 (2019-01-25)
Infections during pregnancy can expose the fetus to microbial Ags, leading to inflammation that affects B cell development. Prenatal fetal immune priming may have an important role in infant acquisition of pathogen-specific immunity. We examined plasma proinflammatory biomarkers, the proportions
Samantha K Greaney et al.
Cancer immunology research, 8(2), 246-254 (2019-12-20)
Whereas systemic IL12 is associated with potentially life-threatening toxicity, intratumoral delivery of IL12 through tavokinogene telseplasmid electroporation (tavo) is safe and can induce tumor regression at distant sites. The mechanism by which these responses are mediated is unknown but is

商品

ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.

实验方案

The ELISpot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level.

Guidelines for various bioassays on the MultiScreen system, which makes it possible to carry out an assay from sample incubation to scintillation counting in a 96-well plate.

相关内容

The ELISpot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level. The popularity of this assay has seen resurgence in recent years as researchers attempt to gain a better understanding of immune responses in a variety of applications. The following protocol is an example of a typical ELISpot assay for quantifying the number cells producing interferon-gamma (IFN-γ) in response to antigen or non-specific activation using phytohemagglutinin (PHA). It may be optimized as necessary for other applications.

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