产品名称
Multiscreen® 96孔板，疏水PVDF薄膜, pore size 0.45 μm, sterile
description
Sterile, white 96-well filter plate with 0.45 um pore size Hydrophobic PVDF membrane for ELISPOT Assays. Comes in a pack of 10.
sterility
sterile
Quality Level
material
Durapore PVDF membrane
flat bottom wells
white acrylic plate
usage
Automated
feature
hydrophobic
lid
packaging
pkg of 10 plates
manufacturer/tradename
MultiScreen®
technique(s)
ELISPOT: suitable
cell based assay: suitable
dot blot: suitable
filtration area
0.28 cm2
plate size
96 wells
well maximum volume
300 μL
working volume
50-250 μL
matrix
Immobilon®-P
pore size
0.45 μm pore size
binding type
high binding surface
compatibility
centrifuge & manifold
shipped in
ambient
Legal Information
Application
- 产双链DNA的浆细胞
- 记忆B细胞和骨髓长寿浆细胞
- 引流淋巴结和脾脏细胞
General description
商品
ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.
实验方案
The ELISpot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level.
Guidelines for various bioassays on the MultiScreen system, which makes it possible to carry out an assay from sample incubation to scintillation counting in a 96-well plate.
相关内容
This provides information about the chemical compatibility of MultiScreen Filter Plates with different reagents.
The ELISpot (Enzyme Linked Immuno-Spot) assay provides an effective method of measuring the antibody or cytokine production of immune cells on the single cell level. The popularity of this assay has seen resurgence in recent years as researchers attempt to gain a better understanding of immune responses in a variety of applications. The following protocol is an example of a typical ELISpot assay for quantifying the number cells producing interferon-gamma (IFN-γ) in response to antigen or non-specific activation using phytohemagglutinin (PHA). It may be optimized as necessary for other applications.
"Under optimal conditions, the enzyme-linked immunosorbent spot (Elispot) assay enables visualization of multiple secretory products from a single responding cell. Thus, the Elispot provides both qualitative (type of immune protein) and quantitative (number of responding cells) information. By virtue of this assay’s unsurpassed sensitivity, frequency analysis of rare cell populations (e.g., antigen-specific responses) which were not possible before are now relatively easy. Recent improvements to the design of multiwell microplates, including use of membranes with reduced background fluorescence, have bolstered the widespread application of Elispot assays. When assay sensitivity, ease of use, and cost are all taken into consideration, the Elispot platform is likely the superior choice for the development of multifunctional T cell assays for the research, therapeutic, and diagnostic communities."
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