Chemical Compatibility Guide for MultiScreen^® Filter Plates

Ratings are based on 100% or concentrated solutions, unless otherwise indicated. Aromatic hydrocarbons (especially ketones, DMF, DMAC, DMSO, THF, acetonitrile) and chlorinated hydrocarbons will attack both membranes and plastics. A chemical listed as NR may be suitable in low concentrations for short exposures, but this must be determined on an individual assay basis.

1. Scintillants should only be used with Barex^® or SAN plates.
2. Surfactants are in general readily usable with all MultiScreen^® plate types. However, before quantitative fluid transfer to a receiver plate, it is essential that the plate be rinsed with non- surfactant containing fluid (e.g., 100 μL PBS) and then the underdrain be thoroughly blotted prior to adding the material which will ultimately be collected. Triton^®-X 100 should not be used with MCE membrane in concentrations greater than 5% (the membrane will appear to lose flow).

1. Scintillants should only be used with Barex^® or SAN plates.
2. Surfactants are in general readily usable with all MultiScreen^® plate types. However, before quantitative fluid transfer to a receiver plate, it is essential that the plate be rinsed with non- surfactant containing fluid (e.g., 100 μL PBS) and then the underdrain be thoroughly blotted prior to adding the material which will ultimately be collected. Triton^®-X 100 should not be used with MCE membrane in concentrations greater than 5% (the membrane will appear to lose flow).

Chemical Compatibility Guide for the MultiScreen^® Vacuum Manifolds

MultiScreen^®HTS and MultiScreen^® Resist Manifolds

The following tables outline the solvents that have been evaluated for compatibility with the various components of the MultiScreen^®HTS vacuum manifold. In general, the manifold will tolerate the same chemicals as the rest of the system.

However, when using concentrated acids, it is important to rinse through the entire manifold at the end of procedures, especially if collection is done through the manifold into a trap, rather than using the collection trays.

Chemical Compatibility Guide for Fixation and Staining

Millicell^® cell culture inserts (single-well, 24-well plates and 96-well plates) are designed to support all fixation, staining and immunostaining procedures in a single device. The 24 and 96-well plates are also automation compatible. The variety of membranes offered in the inserts allows the fixed and stained cells to be visualized by stereoscopic microscopy, phase contrast microscopy, or fluorescent methods.

The majority of staining procedures employ a fixation step first. Fixation is required to stabilize sub-cellular morphology and prevent degradation of antigens during subsequent staining procedures. Consult the following table for chemical compatibility information with common fixative chemicals and stains.

General Considerations

• If the chemicals are compatible with the membrane but not the polystyrene housing, remove the membrane from the housing before adding the chemical.
• Unless otherwise stated, the chemicals listed are at maximum concentration. If the plastic housing and/or membranes are not compatible with the maximum concentration, they might be compatible at lower concentration.