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Merck
CN

MABT401

抗-润滑素抗体/蛋白聚糖4,克隆9G3

clone 9G3, from mouse

别名:

Proteoglycan 4, Lubricin, Megakaryocyte-stimulating factor, Superficial zone proteoglycan, Proteoglycan 4 C-terminal part

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
9G3, monoclonal
Application:
immunohistochemistry
western blot
Species reactivity:
rat, human
Citations:
22
Technique(s):
immunohistochemistry: suitable (paraffin)
western blot: suitable
Uniprot accession no.:
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产品名称

抗-润滑素抗体/蛋白聚糖4,克隆9G3, clone 9G3, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

9G3, monoclonal

species reactivity

rat, human

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

Gene Information

human ... PRG4(10216)

Analysis Note

通过蛋白质印迹法对润滑素纯化蛋白进行评价。

蛋白质印迹分析:0.02 µg/mL该抗体在1 µg润滑素纯化蛋白中检测到了润滑素/蛋白聚糖4。

Application

免疫组织化学分析:一个代表性批次以1:1,000稀释度在人肝脏和人心脏组织中检测到润滑素/蛋白聚糖4。
免疫组织化学分析:一个代表性批次在关节软骨组织中检测到润滑素/蛋白聚糖4(Elsaid,K.A,et al.(2012).Osteoarthritis and Cartilage.20:940-948)。
免疫组织化学分析:一个代表性批次在大鼠动脉软骨组织中检测到润滑素/蛋白聚糖4(Jay,G.D,et al.(2012).Arthritis & Rheumatism.64(4):940-948)。
免疫组织化学分析:一个代表性批次在大鼠的关节软骨中检测到润滑素/蛋白聚糖4
(手稿正在准备中)。
蛋白质印迹分析:一个代表性批次在从患者滑液中纯化的润滑素中检测到润滑素/蛋白聚糖4(手稿正在准备中)。
经验证,该抗润滑素抗体/蛋白聚糖4(克隆9G3)可用于蛋白质印迹法和免疫组织化学(石蜡)检测润滑素/蛋白聚糖4。

General description

称为润滑素/蛋白聚糖4的蛋白质是关节中对关节功能至关重要的重要分泌蛋白,因此也是关节疾病研究的重点。润滑素/蛋白聚糖4在边界润滑中起作用,并通过控制滑膜细胞的滑膜生长和抑制细胞粘附到软骨表面来防止软骨从滑液中沉积。在发育过程中,润滑素/蛋白聚糖4还充当造血和内皮细胞谱系的生长因子,并且润滑素/蛋白聚糖4对于眼睛等组织中适当的屏障和润滑发展似乎很重要。润滑素/蛋白聚糖4在关节和关节边界附近高表达,但也可以在脑、心脏、肺、肾和其他组织中发现。润滑素/蛋白聚糖4具有几种亚型,它们的细胞和组织表达不同。亚型C和D是表达最广泛的形式。最后,由于其对表面的影响,正在组织工程和人工软骨开发中检查润滑素/蛋白聚糖4作为关节疾病的治疗方法。
观测分子量〜220 kDa。该蛋白质的计算分子量为151 kDa,在145 kDa左右观察到许多亚型,并且由于糖基化而可能更高。

Immunogen

对应于人润滑素/蛋白聚糖4的重组蛋白。

Other Notes

浓度:请参考特定批次的数据表。

Physical form

形式:纯化

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存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Elizabeth Feeney et al.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 37(5), 1071-1079 (2019-03-13)
The objective of this study was to examine temporal variations in synovial fluid composition and lubrication following articular fracture. Post-traumatic osteoarthritis (PTOA) was induced by creating an osteochondral fracture in the middle carpal joint of four horses while the contralateral
Allen Khakshooy et al.
Bioinformation, 13(10), 343-346 (2017-11-23)
Lubricin is a synovial glycoprotein that contributes to joint lubrication. We propose the hypothesis that lubricin is a key modulator of the psychoneuroendocrine-osteoimmune interactome, with important clinical relevance for osteoarthritic pathologies. We consider a variety of neuroendocrine-immune factors, including inflammatory
Roman J Krawetz et al.
NPJ Regenerative medicine, 7(1), 32-32 (2022-06-25)
The wound healing response is one of most primitive and conserved physiological responses in the animal kingdom, as restoring tissue integrity/homeostasis can be the difference between life and death. Wound healing in mammals is mediated by immune cells and inflammatory
Zhong Li et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 9(21), e2105909-e2105909 (2022-04-19)
Diseases of the knee joint such as osteoarthritis (OA) affect all joint elements. An in vitro human cell-derived microphysiological system capable of simulating intraarticular tissue crosstalk is desirable for studying etiologies/pathogenesis of joint diseases and testing potential therapeutics. Herein, a
Amy C Madl et al.
ACS applied materials & interfaces, 14(16), 18016-18030 (2022-04-14)
Dry eye disease (DED) affects more than 100 million people worldwide, causing significant patient discomfort and imposing a multi-billion-dollar burden on global health care systems. In DED patients, the natural biolubrication process that facilitates pain-free blinking goes awry due to

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