Anti-PCSK9 Antibody, clone 9B3.1

Proprotein convertase subtilisin/kexin type 9 (UniProt Q8NBP7; also known as NARC-1, Neural apoptosis-regulated convertase 1, PC9, Proprotein convertase 9, Subtilisin/kexin-like protease PC9) is encoded by the PCSK9 (also known as HCHOLA3, LDLCQ1, NARC1, PSEC0052) gene (Gene ID 255738) in human. PCSK9 is a proprotein convertase expressed in the hepatocyte and to a lesser extent also in the intestine and kidney. PCSK9 is produced as a precusor protein with a signal peptide (a.a.1-30) and a propeptide sequence (a.a. 31-152) that undergo posttranslational autocleavage to yield the mature PCSK9 protein (a.a. 153-692). PCSK9 utilizes its proteolytic activity only in autocleavage during protein maturation, a process that is necessary for its secretion. Once secreted into the plasma, PCSK9 binds LDL receptor (LDL-R), causing LDL-R endocytosis and lysosomal degradation, effectively reducing cell surface LDL-R population. Both PCSK9 and LDL-R are transcribed by sterol-responsive element-binding protein 2 (SREBP2) in response to lowered cellular cholesterol level, allowing enhanced, but not unfettered cellular cholesterol uptake by LDL-R. PCSK9 also binds very low density lipoprotein (VLDL) receptor and apolipoprotein E receptor 2, and thereby interferes with uptake of circulating VLDL particles and apolipoproteins.

Clone 9B3.1 detects the precursor and mature forms of human PCSK9 (NARC-1) by targeting an epitope within the N-terminal region of the mature sequence.

GST-tagged recombinant human mature NARC-1 (PCSK9) N-terminal fragment.

Detect PCSK9 using this mouse monoclonal Anti-PCSK9, clone 9B3.1 Antibody, Cat. No. MABS434, validated for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected NARC-1 (PCSK9) in human colon cancer, normal kidney and prostate tissue sections.

Western Blotting Analysis: A 1:500 dilution from a representative lot detected NARC-1 (PCSK9) in 10 µg of human colon tumor tissue lysate.

Research Category
Signaling

Evaluated by Western Blotting in SW480 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this hybridoma culture supernatant detected NARC-1 (PCSK9) in 10 µg of SW480 human colon adenocarcinoma cell lysate.

~75/60 kDa observed. 74.29 kDa (prepro-form; a.a. 1-692), 71.05 kDa (pro-form; a.a. 31-692), and 57.29 kDa (mature form; a.a. 153-692) calculated. Uncharacterized bands may be observed in some lysate(s).

Unpurified

Mouse IgG2a hybridoma culture supernatant without azide.

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.