biological source
mouse
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
2B11.1, monoclonal
species reactivity
human
technique(s)
immunohistochemistry: suitable (paraffin), western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... HAS2(3037)
General description
Hyaluronan synthase 2 (HAS2) is also known as Hyaluronate synthase 2, or Hyaluronic acid (HA) synthase 2. As the name suggests, HAS2 is involved in hyaluronan/hyaluronic acid synthesis. Hyaluronan synthase 2 is expressed in fibroblasts. Chromosomal aberrations involving Hyaluronan synthase 2 may be a cause of lipoblastomas, usually diagnosed in children.
~ 64 kDa observed
Immunogen
Epitope: Cytoplasmic domain
GST-tagged recombinant protein corresponding to the cytoplasmic domain of human Hyaluronan synthase 2.
Application
Detect Hyaluronan synthase 2 using this mouse monoclonal antibody, Anti-Hyaluronan synthase 2 Antibody, clone 2B11.1 validated for use in western blotting & IHC (Paraffin).
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
Western Blotting Analysis: 2.0 µg/mL from a representative lot detected Hyaluronan synthase 2 in 10 µg of K562 cell lysate.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Hyaluronan synthase 2 in human breast adenocarcinoma cell tissue.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Hyaluronan synthase 2 in human breast adenocarcinoma cell tissue.
Biochem/physiol Actions
This antibody recognizes the cytoplasmic domain of Hyaluronan synthase 2.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
MRC-5 cell lysate
MRC-5 cell lysate
Evaluated by Western Blotting in MRC-5 cell lysate.
Western Blotting Analysis: 0.2 µg/mL of this antibody detected Hyaluronan synthase 2 in 200 µg of MRC-5 cell lysate.
Western Blotting Analysis: 0.2 µg/mL of this antibody detected Hyaluronan synthase 2 in 200 µg of MRC-5 cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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