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MAB5492

Sigma-Aldrich

抗-亨廷顿蛋白抗体,a.a.1-82

ascites fluid, clone 2B4, Chemicon®

别名:

Anti-Huntingtin, Anti-Huntingtin Antibody, Detection Antibody

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About This Item

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物来源

mouse

质量水平

抗体形式

ascites fluid

抗体产品类型

primary antibodies

克隆

2B4, monoclonal

种属反应性

human

制造商/商品名称

Chemicon®

技术

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

同位素/亚型

IgG1

NCBI登记号

UniProt登记号

运输

dry ice

靶向翻译后修饰

unmodified

基因信息

特异性

与亨廷顿蛋白(氨基酸1-82)反应。该抗体可识别野生型和突变型亨廷顿蛋白。

免疫原

表位:a.a.1-82
重组人亨廷顿蛋白,氨基酸1-82。

应用

抗-亨廷顿蛋白抗体,a.a.1-82是用于ELISA、IC、IH&WB的抗亨廷顿蛋白的抗体。
研究子类别
神经退行性疾病
研究类别
神经科学
蛋白质印迹:1:500-1:5,000

免疫细胞化学(1): 1:500-1:5,000

免疫组织化学(1,2): 1:500-1:5000

ELISA:1:500-1:5,000

最佳工作稀释度必须由最终用户确定。

目标描述

348 kDa

外形

不含防腐剂的腹水。
未纯化

储存及稳定性

自收到之日起在-20°C可稳定保存1年。分装保存以避免反复冻融。为了最大程度地回收产品,需在融化后和取下盖子之前将原始样品管进行离心。

分析说明

对照
正常人大脑皮层裂解物,来自HD或野生型小鼠的小鼠大脑皮层样品

其他说明

浓度:关于批次特定浓度请参见检验报告。

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

除非我们的目录或产品随附的其他公司文件中另有说明,否则我们的产品预期仅用于研究用途,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或对人类或动物的任何类型的消费或应用。

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WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


分析证书(COA)

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Sophie Vieweg et al.
The Journal of biological chemistry, 291(23), 12074-12086 (2016-03-24)
The first exon of the Huntingtin protein (Httex1) is one of the most actively studied Htt fragments because its overexpression in R6/2 transgenic mice has been shown to recapitulate several key features of Huntington disease. However, the majority of biophysical
Sean M DeGuire et al.
The Journal of biological chemistry, 293(48), 18540-18558 (2018-09-07)
Huntington's disease is a fatal neurodegenerative disorder resulting from a CAG repeat expansion in the first exon of the gene encoding the Huntingtin protein (Htt). Phosphorylation of this protein region (Httex1) has been shown to play important roles in regulating
Phosphorylation of mutant huntingtin at serine 116 modulates neuronal toxicity.
Watkin, EE; Arbez, N; Waldron-Roby, E; O'Meally, R; Ratovitski, T; Cole, RN; Ross, CA
Testing null
Gennady Ermak et al.
The Journal of biological chemistry, 284(18), 11845-11853 (2009-03-10)
Our work suggests an important new link between the RCAN1 gene and Huntington disease. Huntington disease is caused by expansion of glutamine repeats in the huntingtin protein. How the huntingtin protein with expanded polyglutamines (mutant huntingtin) causes the disease is
Jack C Reidling et al.
Stem cell reports, 10(1), 58-72 (2017-12-14)
Huntington's disease (HD) is an inherited neurodegenerative disorder with no disease-modifying treatment. Expansion of the glutamine-encoding repeat in the Huntingtin (HTT) gene causes broad effects that are a challenge for single treatment strategies. Strategies based on human stem cells offer

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