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Merck
CN

MAB3328

抗MMP-14抗体，催化域，克隆LEM-2 / 15.8

clone LEM-2/15.8, Chemicon®, from mouse

别名:

MT1-MMP

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关于此项目

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41

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生物来源

mouse

质量水平

100

偶联物

unconjugated

抗体形式

purified immunoglobulin

抗体产品类型

primary antibodies

克隆

LEM-2/15.8, monoclonal

种属反应性

mouse, human

包装

antibody small pack of 25 μg

制造商/商品名称

Chemicon®

技术

ELISA: suitable
immunoprecipitation (IP): suitable
western blot: suitable

同位素/亚型

IgG1κ

NCBI登记号

NM_004995.2

UniProt登记号

P50281

运输

ambient

储存温度

2-8°C

靶向翻译后修饰

unmodified

基因信息

human ... MMP14(4323)

相关类别

一般描述

MT1-MMP 在内皮细胞迁移和基质重塑中起着重要作用。 尽管 MT1-MMP 在内皮细胞运动中的作用尚未完全阐明，但它的活性似乎在血管生成反应期间调节内皮的迁移、侵袭和毛细管的形成（Galvez，2001）。 Mt1-MMP 在炎症过程中的单核细胞再生中也起着关键作用（Salomon，2005）。

免疫原

合成肽：催化结构域内的氨基酸序列 218-233
表位：催化结构域

应用

使用该抗 MMP-14 抗体（催化域，克隆 LEM-2/15.8，经验证可用于 ELISA、IP & WB）检测 MMP-14。
研究子类别
MMPs & TIMPs
研究类别
细胞结构
蛋白质印迹

免疫组化：冷冻和石蜡包埋的组织

免疫荧光

流式细胞术

封闭：10-15μg/mL

最佳工作稀释度必须由最终用户确定。

生化/生理作用

LEM-2/15.8 与人 MT1-MMP 发生反应，并与小鼠标本产生交叉反应。 该抗体是针对 MT1-MMP 的催化结构域生成的，能够抑制酶的活性。

外形

形式：纯化的
通过蛋白 A 层析纯化的免疫球蛋白。 溶于 0.2M 磷酸盐，0.25M NaCl，pH 7.6 的液体，含有 0.1％ 叠氮化钠。

制备说明

自收到之日起，置于 2° 至 8°C 最多可保存 12 个月。

其他说明

浓度：请参考批次特异性浓缩物的分析证书。

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

分析证书(COA)

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G Daniel Grass et al.
Journal of cell science, 125(Pt 3), 777-788 (2012-03-06)
A defining feature of malignant tumor progression is cellular penetration through the basement membrane and interstitial matrices that separate various cellular compartments. Accumulating evidence supports the notion that invasive cells employ specialized structures termed invadopodia to breach these structural barriers.
J K Björk et al.
Oncogene, 35(14), 1770-1784 (2015-06-30)
Heat-shock factors (HSFs) are key transcriptional regulators in cell survival. Although HSF1 has been identified as a driver of carcinogenesis, HSF2 has not been explored in malignancies. Here, we report that HSF2 suppresses tumor invasion of prostate cancer (PrCa). In
Charles Marusak et al.
Pharmacological research, 113(Pt A), 515-520 (2016-10-21)
MT1-MMP and MMP2 have been implicated as pro-tumorigenic and pro-metastatic factors in a wide variety of cancers including melanoma. We have previously demonstrated that MT1-MMP is highly expressed in melanoma where it promotes melanoma cell invasion and metastasis in part
Karina Di Gregoli et al.
Arteriosclerosis, thrombosis, and vascular biology, 34(9), 1990-2000 (2014-07-06)
Our recent studies have highlighted membrane type-1 matrix metalloproteinase (MMP)-14 as a selective marker for an invasive subset of macrophages potentially related to atherosclerotic plaque progression. Moreover, colony stimulating factors (CSF) may exert divergent effects on macrophage MMP expression, possibly
Susan H Taylor et al.
eLife, 4, e09345-e09345 (2015-09-22)
Type I collagen-containing fibrils are major structural components of the extracellular matrix of vertebrate tissues, especially tendon, but how they are formed is not fully understood. MMP14 is a potent pericellular collagenase that can cleave type I collagen in vitro.
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Fluorescent Gelatin Degradation Assays for Investigating Invadopodia Formation

QCM™ Gelatin Invadopodia Assays provide optimized materials and protocols to enable reproducible analysis of invadopodia in invasive tumor cells (Catalog No. ECM670 for green fluorescence, Catalogue No. ECM671 for red fluorescence). Reagents are provided for coating glass culture surfaces with fluorescent matrix and for colocalizing the actin cytoskeleton and nuclei with invadopodial degradation sites. This assay may also be used for assessing the activity of inhibitors and promoters of invadopodia formation and function. Furthermore, different cell types and individual cells in heterogeneous populations may be analyzed for invasive potential. Finally, the assay kits provide troubleshooting suggestions, recommendations for coating on multiple substrate formats, and example studies in several assay systems (e.g., various cell types, time-course studies, degradation modulation).

MOUSE ANTI-MMP-14 [MT1-MMP] MONOCLONAL ANTIBODY

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