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Merck
CN

MAB1976Z

Anti-Integrin αVβ3 Antibody

CHEMICON®, mouse monoclonal, LM609

别名:

VitronectinReceptor, CD51/CD61, MAB1976

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UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

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产品名称

抗整合素αVβ 3抗体，克隆LM609，无叠氮化物, clone LM609, Chemicon®, from mouse

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

LM609, monoclonal

species reactivity

human, canine, pig, rabbit, bovine, chicken, monkey, avian

should not react with

mouse, rat

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

isotype

IgG1

suitability

not suitable for immunohistochemistry (Paraffin)

NCBI accession no.

NM_002210.2

UniProt accession no.

P06756

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... ITGAV(3685)

相关类别

Analysis Note

对照
阳性对照：人骨髓瘤细胞系M21和MoαV（Chen，1995）。
阴性对照：Mo细胞（该细胞系来源于M21，但不表达整联蛋白αV

Chen，1995）

Application

流式细胞术：100μL中每0.5 x 10e6个细胞1-3 μg LM609（Smith，1996）。

免疫沉淀：特异性免疫沉淀整联蛋白αvβ3复合物，当在还原条件下通过SDS-PAGE分离时，产生对应于αv亚基的约130 kDa条带和对应于β3亚基的105 kDa条带（Cheresh，1987）。

免疫荧光：5-10 μg/mL，用于用丙酮固定的经bFGF处理的6 mm鸡胚绒毛尿囊膜冷冻切片的染色（Brooks，1994）。LM609对4%多聚甲醛固定、冷冻的组织和细胞也有效。但对石蜡包埋组织切片的免疫组织化学染色无效。

在10-25 μg/mL浓度下抑制细胞对玻连蛋白包被表面的粘连。

最佳的工作稀释度和方案必须由最终用户确定。
研究子类别
整联蛋白
研究类别
细胞结构
该抗整联蛋白αVβ3抗体（克隆LM609，无叠氮化物）经验证可用于FC、IH、IP、FUNC检测整联蛋白αVβ3。

Biochem/physiol Actions

与玻连蛋白受体αvβ3复合物（一种RGD定向的粘附受体）反应。已经证明LM609对人黑素瘤细胞系（M21）对玻连蛋白、纤维蛋白原和血管性假血友病因子以及含有肽的合成RGD的阻断粘连（Cheresh，1987）。在鸡胚绒毛尿囊膜中，LM609显示阻断bFGF和TNFα诱导的血管生成，但对已有的血管没有作用（Brooks，1994）。尽管LM609阻断细胞粘附在含有RGD的配体上，但它不直接与RGD结合位点相互作用。相反，LM609似乎是整联蛋白αvβ3的变构抑制剂，其与由αV和β3亚基的翻译后关联性产生的构象表位结合。

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

General description

整联蛋白αV的分子量：125-135kda
细胞粘附受体的整联蛋白家族由至少16种膜相关的异二聚体组成，由以非共价方式缔合的α和β亚基组成。整联蛋白家族的结构和功能多样性基于各个α和β亚基的配对能力。整联蛋白受体与其各自配体之间这些分子相互作用的关键是对Arg-Gly-Asp（RGD）序列的识别，已知该序列存在于细胞外基质成分包括纤连蛋白、玻连蛋白、胶原蛋白、纤维蛋白原和血管性假血友病因子中（Cheresh，1991）。整联蛋白在血管增殖、粘附和伤口修复中的作用已被充分证明。粘附受体整联蛋白αvβ3似乎在生长的血管上选择性表达，并已被确定为血管生成血管组织的标志物（Brooks，1994）。由于其可参与血管生成，整联蛋白αVβ3是研究最多的整联蛋白受体之一。

Immunogen

从人黑素瘤细胞系M21纯化的粘附受体（Cheresh，1987）。

Other Notes

浓度：请参考批次特异性浓缩物的分析证书。

Physical form

形式：纯化
纯化的蛋白 A
蛋白 A 纯化的小鼠免疫球蛋白，溶于 20 mM 磷酸钠，250 mM NaCl，pH.7.6，不含防腐剂的溶液中。

Preparation Note

以未稀释的等分试样保存于2–8°C下6个月。

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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存储类别

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Abdullah Karadag et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 21(10), 1627-1636 (2006-09-26)
BMSCs migrate through matrix barriers and differentiate into osteoblasts. BSP enhances osteogenic cell migration through basement membrane and collagen matrices in vitro by localizing MMP-2 on the cell surface through alpha(v)beta(3)-integrin. The specific mechanisms by which bone marrow stromal cells
Martin Michaelis et al.
The American journal of pathology, 180(4), 1370-1377 (2012-01-31)
The influences of cytotoxic drugs on endothelial cells remain incompletely understood. Herein, we examined the effects of chemotherapeutic agents in experimental angiogenesis models and analyzed vessel densities in clinical neuroblastoma tumor samples. Cisplatin (20 to 500 ng/mL), doxorubicin (4 to
Junmin Lee et al.
Acta biomaterialia, 42, 46-55 (2016-07-05)
Mesenchymal stem cells (MSCs) can differentiate into multiple lineages through guidance from the biophysical and biochemical properties of the extracellular matrix. In this work we conduct a combinatorial study of matrix properties that influence adipogenesis and neurogenesis including: adhesion proteins
Paul A Rupp et al.
Development (Cambridge, England), 131(12), 2887-2897 (2004-05-21)
A major challenge confronting developmental cell biologists is to understand how individual cell behaviors lead to global tissue organization. Taking advantage of an endothelial cell-specific marker and scanning time-lapse microscopy, we have examined the formation of the primary vascular pattern
Attenuation of retinal endothelial cell migration and capillary morphogenesis in the absence of bcl-2.
Kondo, S; Tang, Y; Scheef, EA; Sheibani, N; Sorenson, CM
American Journal of Physiology. Cell Physiology null
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