跳转至内容
Merck
CN

MAB1864-I

抗-α-微管蛋白抗体,酪氨酸化,克隆YL1/2

clone YL 1/2, from rat

别名:

Tubulin alpha-1A chain, Alpha-tubulin 3, Tubulin B-alpha-1, Tubulin alpha-3 chain, alpha-Tubulin, tyrosinated

登录 查看组织和合同定价。

选择尺寸


关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
技术服务
需要帮助?我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助
技术服务
需要帮助?我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助

产品名称

抗-α-微管蛋白抗体,酪氨酸化,克隆YL1/2, clone YL 1/2, from rat

biological source

rat

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

YL 1/2, monoclonal

species reactivity

yeast, porcine, rat, chicken, bovine, human, mouse

technique(s)

ELISA: suitable
electron microscopy: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
radioimmunoassay: suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

Gene Information

human ... TUBA1A(7846)

Physical form

纯化的蛋白G
在含 0.1 M Tris-甘氨酸(pH 7.4),150 mM NaCl 和 0.05% 叠氮化钠的缓冲液中的纯化的大鼠单克隆 IgG2aκ 抗体。
形式:纯化

Preparation Note

自发运之日起,在 2-8°C 条件下可稳定保存1年

Analysis Note

通过蛋白质印迹在 A431 细胞裂解物中进行了评估。

蛋白质印迹分析:1.0 µg/mL 的该抗体在 10 µg A431 细胞裂解物中检测到酪氨酸化的 α-微管蛋白。

Application

研究子类别
粘附(CAMs)
研究类别
细胞结构
蛋白质印迹分析:1.0 µg/mL代表性批次在 10 µg C2C12细胞裂解物中检测到酪氨酸化α-微管蛋白。
免疫细胞化学分析:4.0µ g/mL代表性批次在A431、HeLa和HUVEC细胞中检测到酪氨酸化α-微管蛋白。
免疫细胞化学分析:代表性批次在酵母中检测到苯丙氨酸化α-微管蛋白的免疫反应性,在HEK293细胞中检测到酪氨酸化α-微管蛋白的免疫反应性(Kilmartin J.V., et al. (1982).J Cell Biol. 93(3):576-582.;Bandyopadhyay, B., et al. (2007).J. Biol. Chem. 282(22):16454-16464)。
免疫组化分析:代表性批次在Hamburger-Hamilton阶段4(HH4)雏鸡胚胎的中线水平切片中检测到微管酪氨酸化α-微管蛋白免疫反应性(Nakaya, Y., et al. (2008).Nat. Cell Biol. 10(7):765-775)。
电子显微镜分析:代表性批次使用粗酵母核被膜制剂检测到微管苯丙氨酸化的α-微管蛋白免疫反应性,并在NIH/3T3细胞中检测到微管酪氨酸化的α-微管蛋白免疫反应性(Wehland, J., and Willingham, M.C.(1983).J Cell Biol. 97(5 Pt 1):1476-1490; Kilmartin J.V., et al. (1982).J Cell Biol. 93(3):576-582)。
ELISA分析:使用猪脑源性微管蛋白对在C-末端有或没有Tyr/Phe的α-微管蛋白肽,通过竞争性ELISA测定克隆YL1/2的抗原性(Wehland, J., et al. (1984).EMBO J. 3(6):1295-1300)。
放射免疫测定法分析:通过放射免疫测定法测定克隆YL1/2对鸡脑微管蛋白和酵母微管蛋白的免疫反应性(Kilmartin J.V., et al. (1982).J Cell Biol. 93(3):576-582)。
这种酪氨酸化的抗α-微管蛋白抗体克隆YL1/2经验证可用于蛋白质印迹,免疫细胞化学,免疫组化,电子显微镜检查,ELISA和放射免疫测定,以检测α-微管蛋白。

Biochem/physiol Actions

与α-微管蛋白在C末端与苯丙氨酸(酵母)或酪氨酸(哺乳动物和鸡)反应(PMID 6204858)。对缺少C末端Phe或Tyr的翻译后修饰的α-微管蛋白不反应。
基于100%的序列同源性,预期可与广泛的物种反应。

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

General description

微管蛋白α-1链(UniProt P09733;也称为α-微管蛋白)由酵母中的TUB1基因(基因ID 854889)编码。微管是动态蛋白丝,参与多种细胞活动,从有丝分裂和转运事件到细胞运动和细胞形状的维持。微管由α-和β-微管蛋白亚基组装而成,这两个亚基都经过翻译后修饰,包括通过羧肽酶活性从α-微管蛋白(哺乳动物中的酪氨酸和酵母中的苯丙氨酸)中去除C末端芳香族氨基酸。由于C端酪氨酸/苯丙氨酸之前是谷氨酸(E)残基,因此脱酪氨酸/脱苯丙氨酸的α微管蛋白也称为Glu-微管蛋白。虽然在哺乳动物中酪氨酸可以通过微管蛋白-酪氨酸连接酶重新连接到Glu-微管蛋白的C末端,但尽管存在与其他生物的微管蛋白-酪氨酸连接酶具有显著同源性的酵母YBR094w基因,但苯丙氨酸并未翻译后添加到酵母中的Glu-微管蛋白中。
观测值〜55 kDa

Immunogen

纯化的酵母微管蛋白
表位:C端

Other Notes

浓度:请参考特定批次的数据表。

未找到合适的产品?  

试试我们的产品选型工具.

存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

A rat monoclonal antibody reacting specifically with the tyrosylated form of alpha-tubulin. II. Effects on cell movement, organization of microtubules, and intermediate filaments, and arrangement of Golgi elements.
Wehland, J; Willingham, MC
The Journal of cell biology null
J Wehland et al.
The EMBO journal, 3(6), 1295-1300 (1984-06-01)
We have characterized the epitope of the rat monoclonal antibody YL 1/2 in detail using synthetic peptides and several alpha-tubulin derivatives. The epitope seems to be provided by the linear sequence spanning the carboxy-terminal residues of tyrosinated alpha-tubulin. By competitive
RhoA and microtubule dynamics control cell-basement membrane interaction in EMT during gastrulation.
Nakaya, Y; Sukowati, EW; Wu, Y; Sheng, G
Nature Cell Biology null
Robert L Douglas et al.
Journal of cell science, 133(13) (2020-06-07)
Trypanosoma brucei, the causative agent of African sleeping sickness, has a flagellum that is crucial for motility, pathogenicity, and viability. In most eukaryotes, the intraflagellar transport (IFT) machinery drives flagellum biogenesis, and anterograde IFT requires kinesin-2 motor proteins. In this
Pierre Romé et al.
The Journal of cell biology, 189(4), 651-659 (2010-05-19)
Aurora A is a spindle pole-associated protein kinase required for mitotic spindle assembly and chromosome segregation. In this study, we show that Drosophila melanogaster aurora A phosphorylates the dynactin subunit p150(glued) on sites required for its association with the mitotic

相关内容

"Mods – modifications – small alterations can have a big impact on form and function. In the motorsports world, stock vehicles are modified to enhance their performance. Modifications, or mods, to the engine, drive train, intake and exhaust components add up to provide phenomenal performance gains that can be measured as horsepower and torque increases, which yield a competitive advantage, and result in reduced run times. In biology, proteins undergo modifications that alter their function. Some proteins require the modifications in order to perform their function effectively, whether it’s a pro-protein that is cleaved to produce an active enzyme, or a protein that is phosphorylated to facilitate a signaling process. Other proteins, such as histones, undergo modifications that regulate gene expression and alter cellular function. There are several post translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination that impact protein function and activity. As a result, the analysis of proteins and their post-translational modifications are particularly important for the study of normal and disease-associated processes. New antibodies to detect phospho Histidines are now available from EMD Millipore."

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系客户支持